MicroRNA-520d-3p inhibits osteosarcoma progression by degradation of Akt1

European Review for Medical and Pharmacological Sciences
T-D HeY-M Wang

Abstract

To detect the expression of microRNA-520d-3p in osteosarcoma tissue and the function on the osteosarcoma cells proliferation. We used qRT-PCR to access microRNA-520d-3p level from 10 cases of osteosarcoma and its adjacent tissues. The osteosarcoma cell lines were screened. The microRNA-520d-3p mimics or inhibitor was transfected into human osteosarcoma cells by liposome method, and the cell proliferation of each group was detected by the CCK8 assay. We used bioinformatics methods to detect and predict the target genes of microRNA-520d-3p. Luciferase reporter assay was utilized to detect the relative luciferase activity between microRNA-520d-3p and Akt1. Meanwhile, after cells were transfected with microRNA-520d-3p mimics, microRNA-520d-3p mimics + OE-Akt1, microRNA-520d-3p inhibitor or microRNA-520d-3p inhibitor + si-Akt1, we detected cell viability using CCK-8 assay, respectively to access the interaction between Akt1 and microRNA-520d-3p. Lowly expressed microRNA-520d-3p in osteosarcoma tissues was observed in comparison with adjacent tissues. After transfecting with microRNA-520d-3p mimics, the viability of MG63 and U-20S cells decreased, which was higher in cells transfecting microRNA-520d-3p inhibitor. Bioinformatics predi...Continue Reading

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