MicroRNA-539 inhibits colorectal cancer progression by directly targeting SOX4

Oncology Letters
Jian ZhaoRui Zhang

Abstract

Colorectal cancer (CRC) is the third most prevalent cancer and the fourth most common cause of cancer-associated mortality in males and females globally. Aberrant expression of microRNA-539 (miR-539) has been reported in multiple types of cancer. However, miR-539 expression, function and underlying mechanisms have not been clearly elucidated in CRC. In the present study, miR-539 expression was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in CRC tissues and cell lines. The effects of miR-539 on CRC cells were further examined in in vitro studies. In addition, the direct targets of miR-539 in CRC were investigated using bioinformatics, luciferase reporter assays, RT-qPCR and western blotting. miR-539 was revealed to be significantly downregulated in CRC cell lines and tissues. Decreased miR-539 expression was associated with lymph node metastasis and tumor-node-metastasis stage in patients with CRC. Functional assays revealed that the rescue of miR-539 expression attenuated CRC cell proliferation and invasion in vitro. Additionally, SRY-box 4 (SOX4) was validated as a direct target gene of miR-539 in CRC. Furthermore, SOX4 was revealed to be upregulated in CRC tissues at the mRNA and protein ...Continue Reading

References

Feb 26, 1999·Nucleic Acids Research·M Wegner
Aug 5, 2000·Brain Research. Molecular Brain Research·M CheungP J Scotting
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Feb 4, 2005·The New England Journal of Medicine·Jeffrey A Meyerhardt, Robert J Mayer
Dec 31, 2005·Nucleic Acids Research·Sam Griffiths-JonesAnton J Enright
Jul 10, 2007·Cancer Research·Kati P PorkkaTapio Visakorpi
Nov 10, 2007·Nucleic Acids Research·Sam Griffiths-JonesAnton J Enright
Dec 17, 2009·Cancer Metastasis Reviews·Sylvia K Shenouda, Suresh K Alahari
Nov 3, 2010·Nucleic Acids Research·Ana Kozomara, Sam Griffiths-Jones
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayDonald Maxwell Parkin
Feb 22, 2012·EMBO Molecular Medicine·Marilena V Iorio, Carlo M Croce
Oct 16, 2012·Seminars in Diagnostic Pathology·Jinru ShiaN Volkan Adsay
Oct 19, 2012·Journal of Biomedical Science·Pai-Sheng ChenMien-Chie Hung
Nov 13, 2013·Cancers·Kankana Bardhan, Kebin Liu
Nov 28, 2013·Nucleic Acids Research·Ana Kozomara, Sam Griffiths-Jones
Feb 25, 2014·Biochimica Et Biophysica Acta·Aristeidis G VaiopoulosAthanasios G Papavassiliou
Jan 15, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kishore GudaJoseph E Willis
Jul 21, 2015·World Journal of Gastrointestinal Oncology·Bhavana KondaLakshmi Rajdev
Jul 25, 2015·Biochemical and Biophysical Research Communications·Lixue Gu, Weiming Sun
Jul 28, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Guangjun LiJingjing Liu
Nov 13, 2015·Cell Biochemistry and Function·Ling-yan LvXing-jie Wang
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 9, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Azza Ali AbdelsatirMohamed H Ahmed
Jan 16, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Baochun WangZhanxiang Xiao
Apr 3, 2016·Journal of Experimental & Clinical Cancer Research : CR·Hongtuan ZhangYong Xu
Apr 14, 2016·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Zheng-Qi BaoDa-Min Chai
May 11, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Liqing ZhouShiyong Xin
Jun 14, 2016·Cancer Cell International·Katrin E TagschererWilfried Roth
Feb 6, 2017·Journal of Biochemical and Molecular Toxicology·Haiping JiangKan Jie

❮ Previous
Next ❯

Citations

Nov 20, 2019·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Fenglin CaiJinhai Tang
Jul 4, 2019·International Journal of Oncology·Josue D MoranCarlos S Moreno
Dec 10, 2019·Clinical & Experimental Metastasis·Padacherri Vethil JishnuShama Prasada Kabekkodu
Jan 26, 2021·Cellular and Molecular Neurobiology·M CrespoM Martín

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.