MicroRNA-610 suppresses the proliferation of human glioblastoma cells by repressing CCND2 and AKT3

Molecular Medicine Reports
Xiaomei MoFenghai Liu

Abstract

Previous studies have shown that microRNA (miR)-610 is crucial in a variety of biological processes in various types of human cancer cells. However, the role of this microRNA in glioblastoma (GBM) is presently unclear. In this study, the role of miR‑610 in cell proliferation was investigated in GBM. It was demonstrated that miR‑610 expression is markedly downregulated in GBM cells and GBM tissues compared with normal human astrocytes (NHAs) and normal brain tissue, respectively. Ectopic expression of miR‑610 reduced the proliferation and anchorage‑independent growth of GBM cells, whereas inhibition of miR‑610 promoted this effect. Bioinformatics analysis further revealed cyclin D2 (CCND2) and AKT3, putative tumor promoters, as potential targets of miR‑610. Data from reporter assays showed that miR‑610 directly binds to the 3'‑untranslated region of CCND2 and AKT3 mRNA, and represses their expression at the transcriptional and translational levels. In conclusion, the data provide compelling evidence that miR‑610 functions as an anti‑onco‑miRNA, which is important in inhibiting cell proliferation in GBM, and its anti‑oncogenic effects are mediated chiefly through direct suppression of CCND2 and AKT3 expression.

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Citations

Aug 28, 2018·Journal of Cellular Physiology·Gui-Ling RenXiao-Ming Meng
Jan 31, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ruizhe WangWen Yuan
Dec 13, 2017·Oncotarget·Guangjing ZhangShijun Feng
Dec 29, 2020·Seminars in Cancer Biology·Md Sahab UddinGhulam Md Ashraf
Mar 17, 2021·Experimental Cell Research·Lei ChengYiwei Liao
Oct 4, 2020·Experimental and Molecular Pathology·Omidvar RezaeiSoudeh Ghafouri-Fard
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qian-Yu LiHongmian Zhao
Jul 20, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wenjie HuZheng Sui

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Methods Mentioned

BETA
PCR
transfections
fluorescence-activated cell sorting

Software Mentioned

SPSS
TargetScan

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