MicroRNA 628 suppresses migration and invasion of breast cancer stem cells through targeting SOS1

OncoTargets and Therapy
Chenghui LinHaomiao Li

Abstract

The purpose of this study is to evaluate the effects of miR-628 on migration and invasion of breast cancer stem cells (CSCs), which are essential for tumor recurrence and metastasis. Quantitative reverse transcription-polymerase chain reaction was used to determine the expression of microRNAs and mRNAs. A subpopulation of CD44+/CD24- breast CSCs were sorted by flow cytometry. Transwell assays were used to evaluate cell migration and invasion. Luciferase reporter assays were performed to verify whether miR-628 targeted SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1). pcDNA3.1(+)-SOS1 was constructed for overexpressing SOS1 after transfection. Compared with primary breast cancer cells, bone metastatic breast cancer cells showed significant downregulation of miR-628. The CD44+/CD24- breast CSC subpopulations in MDA-MB-231 and MCF-7 cell lines were analyzed and sorted. Transfection with an miR-628 mimic significantly suppressed the migration and invasion of these breast CSCs by targeting SOS1, which plays an essential role in epithelial-to-mesenchymal transition. Overexpression of SOS1 rescued miR-628-mediated migration and invasion by upregulating Snail and vimentin, and downregulating E-cadherin. miR-628 suppressed migrat...Continue Reading

Citations

Jan 30, 2020·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Zhiqun ZhangLizhong Du
Oct 11, 2019·Cells·Mohammad Mijanur RahmanTrygve O Tollefsbol
Jul 28, 2020·Journal of Oncology·Zhiguang Yang, Zhaoyu Liu
Jul 17, 2020·Non-coding RNA·Jun Sheng Wong, Yoke Kqueen Cheah
Sep 24, 2020·Diagnostics·Clarence Ching Huat KoGeok Chin Tan
Jul 2, 2020·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Beili ChenQin Zhou
Jan 14, 2021·International Journal of Molecular Sciences·Hsing-Ju Wu, Pei-Yi Chu
Nov 3, 2020·Non-coding RNA Research·Oscar Raul Fajardo-RamirezIvan Alberto Marino-Martinez
Apr 18, 2021·Stem Cell Research & Therapy·Kai Song, Maryam Farzaneh
Apr 24, 2021·Frontiers in Physiology·Isabel Fuentes-Calvo, Carlos Martinez-Salgado

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Methods Mentioned

BETA
nucleotide exchange
fluorescence-activated cell sorting
protein assay
electrophoresis
X-ray
transfection
PCR
flow cytometry

Software Mentioned

Image Pro - Plus

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