microRNA-637 promotes apoptosis and suppresses proliferation and autophagy in multiple myeloma cell lines via NUPR1.

FEBS Open Bio
Xuanxin ChenJianbin Chen

Abstract

Multiple myeloma (MM) is a heterogeneous disease with poor prognosis. Increasing evidence has revealed that microRNAs (miRNAs) are strongly associated with the pathogenesis and progression of MM. Here, we investigated the role of microRNA-637 (miR-637) in MM to identify potential therapeutic targets. We measured the expression of miR-637 in bone marrow samples of MM patients and MM cell lines by quantitative real-time PCR and western blot. The effect of miR-637 on proliferation and apoptosis of MM primary cells was also investigated. Analyses of four bioinformatics databases showed that miR-637 is associated with nuclear protein 1 (NUPR1) in MM cells, which was confirmed by luciferase reporter assay. We found that the overexpression of miR-637 suppressed the development of MM. miR-637 mimics increased the levels of Bax, cleaved caspase 3, and P62, and decreased the levels of Bcl2 and LC3. Additionally, luciferase reporter assays were performed to demonstrate that NUPR1 is the main target of miR-637 in MM cells. Overexpression of NUPR1 reversed the effects of miR-637 mimics in MM cells. Our results suggest that miR-637 inhibits cell proliferation and autophagy, and promotes apoptosis in MM cells by targeting NUPR1. Our findings ...Continue Reading

References

May 20, 2015·Current Opinion in Genetics & Development·Raphaël Szalat, Nikhil C Munshi
Jun 14, 2016·American Journal of Hematology·S Vincent Rajkumar
Jul 2, 2016·Journal of Hematology & Oncology·Leonard Naymagon, Maher Abdul-Hay
Dec 10, 2017·Hematology·Heinz Ludwig, Niklas Zojer
Feb 24, 2018·The International Journal of Biochemistry & Cell Biology·Qingling YuanXiubo Lu
Aug 14, 2018·International Immunopharmacology·Xiaozhi BaiDahai Hu
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Jul 8, 2020·Maturitas·Despina FotiouMeletios Athanasios Dimopoulos

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Methods Mentioned

BETA
transfection
flow cytometry
PCR
Assay
electrophoresis
transfecting

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