MicroRNA-655-3p functions as a tumor suppressor by regulating ADAM10 and β-catenin pathway in Hepatocellular Carcinoma

Journal of Experimental & Clinical Cancer Research : CR
Gang WuYing Cheng

Abstract

Increasing evidence suggests that microRNAs (miRNAs) play critical roles in malignant transformation, tumor progression and metastasis. Aberrant miR-655-3p expression has been associated with several cancers. However, the role and underlying mechanism of miR-655-3p in the development of hepatocellular carcinoma (HCC) remains unclear. MiR-655-3p expression was detected by quantitative RT-PCR (qRT-PCR) in human HCC tissues and cell lines. Cell proliferation was investigated using MTT and colony formation assays, and cell migration and invasion abilities were evaluated by transwell assay. ADAM10 protein expression was detected by immunohistochemical assay. The target gene and downstream of miR-655-3p were determined by qRT-PCR, western blot and dual-luciferase reporter assays. miR-655-3p was significantly down-regulated in HCC tissues and HCC cell lines. Low miR-655-3p expression was negatively related to tumor size, portal vein tumor thrombosis (PVTT) status, TNM stage and metastasis status. In addition, miR-655-3p overexpression and depletion decreased and increased HCC cell proliferation, migration and invasion, respectively. Moreover, ADAM10 was identified as a direct target of miR-655-3p, and miR-655-3p down-regulated E-cadhe...Continue Reading

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Citations

Nov 8, 2018·Journal of Experimental & Clinical Cancer Research : CR·Ke-Ting QueZuo-Jin Liu
May 7, 2019·Molecular Medicine Reports·Xueyan FuWenqing Wang
Mar 28, 2020·Journal of Molecular Neuroscience : MN·Mehrdokht MazdehSoudeh Ghafouri-Fard
Mar 22, 2020·Journal of Cellular Physiology·Reza Jafarzadeh-EsfehaniAmir Avan
Sep 28, 2018·Scientific Reports·Jinha HwangKyoung-Mee Kim
May 16, 2019·Journal of Cellular and Molecular Medicine·Xu ZhouKeshen Li

Related Concepts

ADAM10 protein, human
CTNNB1 protein, human
MIRN655 microRNA, human
ADAM10 Protein
Cell Motility
Liver Carcinoma
Malignant Neoplasm of Liver
Cell Surface Proteins
Neoplasm Invasiveness
TNM Staging System

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