MicroRNA-744 Inhibits Cellular Proliferation and Invasion of Colorectal Cancer by Directly Targeting Oncogene Notch1.

Oncology Research
Jian Shen, Minzhe Li

Abstract

Accumulated studies have strongly implicated aberrantly expressed microRNAs (miRNAs) in carcinogenesis and cancer progression of various cancers, including colorectal cancer (CRC). Hence, a comprehensive study of miRNAs and their association with CRC may be a promising therapeutic method for patients with this malignancy. MicroRNA-744 (miR-744) is abnormally expressed in several types of human cancer. Thus far, little is known about the expression, biological roles, and exact mechanisms of miR-744 in CRC. Thus, the present study measured the expression level of miR-744 and investigated its roles and associated molecular mechanisms in CRC. This study demonstrated that miR-744 expression was significantly underexpressed in CRC tissues and cell lines. Low miR-744 expression was positively associated with lymphatic metastasis and TNM stage. Functional experiments revealed that miR-744 overexpression obviously inhibited the proliferation and invasion of CRC cells. Furthermore, Notch1 was identified as a direct target of miR-744 in CRC. Moreover, the inhibition of Notch1 phenocopied the inhibitory effects of miR-744 overexpression on CRC cells. Restored Notch1 expression markedly rescued the tumor-suppressive effects of miR-744 overe...Continue Reading

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Citations

May 16, 2019·Experimental and Therapeutic Medicine·Huixiang YaoJinshui Zhu
Mar 19, 2020·Molecular Medicine Reports·Wei YuXiao-Yun Pan
Jun 20, 2020·The Kaohsiung Journal of Medical Sciences·Lai-Gang ZhaoQin-Fen Li
Mar 23, 2021·Breast Disease·Farah Hady El KilanyMohamed Zakaria Gad

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Methods Mentioned

BETA
surgical resection
PCR
transfection
Assay
electrophoresis

Software Mentioned

miRanda
SPSS
TargetScan

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