MicroRNA-886-3P functions as a tumor suppressor in small cell lung cancer

Cancer Biology & Therapy
Jie ShenLv-Hua Wang

Abstract

Small cell lung cancer (SCLC) is a highly aggressive disease and miRNAs may play an important role in modulating SCLC progression. We have previously screened 924 miRNAs and found that miR-886-3P was negatively associated with SCLC survival. In the current study, we further investigated the role of miR-886-3P mimic in regulating SCLC cell phenotypic alteration in vitro and xenograft tumor formation in vivo. We found that transfection of miR-886-3P mimic significantly inhibited SCLC cell proliferation, migration, and colony formation, and induced mesenchymal-epithelial transition (MET) by suppressing TGF-ß1 synthesis in vitro. Furthermore, intra-tumor injection of miR-886-3P mimic lead to necrosis and suppression of tumor invasion to the surrounding tissue in the subcutaneous xenograft tumor, and intra-vein injection of miR-886-3P mimic suppressed xenograft lung cancer growth in vivo. These findings suggested that miR-886-3P functions as a tumor suppressor in SCLC and thus, it might be a potential therapeutic molecule in the treatment of lung cancer.

References

Aug 22, 2002·Nature Reviews. Cancer·Jean Paul Thiery
Sep 18, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Patrick MickeRoland Buhl
Apr 17, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gregory M M VideticEdward W Yu
Jun 9, 2006·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M V CéspedesR Mangues
Sep 26, 2008·The New England Journal of Medicine·Roy S HerbstScott M Lippman
Mar 20, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Wei-Chih TsaiAnn-Ping Tsou
Jun 29, 2010·Cancer Treatment Reviews·Young Hak Kim, Michiaki Mishima
Oct 30, 2012·Molecular Oncology·Martin D Jansson, Anders H Lund
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Mar 10, 2016·Translational Lung Cancer Research·Gabriela Alvarado-Luna, Daniela Morales-Espinosa
Jul 12, 2016·Biochemical and Biophysical Research Communications·Ichwaku RastogiNeelu Puri
Nov 14, 2016·JAMA : the Journal of the American Medical Association·Julie C Lumeng
Jan 23, 2017·The Annals of Thoracic Surgery·Asishana A OshoAshok Muniappan
Jan 27, 2017·International Journal of Molecular Sciences·Cristina BorziOrazio Fortunato
Feb 10, 2017·Anticancer Research·Hind RafeiImad Tabbara

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Citations

Feb 14, 2019·Thoracic Cancer·Huizhu GanButong Sun
Mar 1, 2020·Experimental and Molecular Pathology·Soudeh Ghafouri-FardMohammad Taheri
Aug 10, 2021·F1000Research·Rafael Sebastián Fort, María Ana Duhagon

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Methods Mentioned

BETA
xenograft
transfection
Protein Assay
PCR
electrophoresis

Software Mentioned

GraphPad Prism
Image
GraphPad

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