MicroRNA-9 Enhanced Cisplatin Sensitivity in Nonsmall Cell Lung Cancer Cells by Regulating Eukaryotic Translation Initiation Factor 5A2

BioMed Research International
Qiaoling PanGuodong Xu

Abstract

We determined the role of microRNA (miR)-9 in regulating cisplatin chemoresistance in nonsmall cell lung cancer (NSCLC) cells. miR-9 and eukaryotic translation initiation factor 5A2 (eIF5A2) levels were examined by reverse transcription-quantitative PCR. Cell Counting Kit-8 and the 5-ethynyl-2'-deoxyuridine (EdU) assay were used to determine the effects of miR-9 mimic or inhibitor on NSCLC cell proliferation and viability, respectively. Bioinformatics was used to analyze the relationship between miR-9 and eIF5A2. Flow cytometry was used to analyze the percentage of apoptotic cells. miR-9 mimic enhanced cisplatin sensitivity, while miR-9 inhibitor produced the opposite result. eIF5A2 was identified as a potential target of miR-9, where miR-9 regulated eIF5A2 expression at mRNA and protein level. miR-9 mimic decreased the expression of eIF5A2 mRNA and protein, while miR-9 inhibitor increased eIF5A2 expression. eIF5A2 knockdown resolved the effects of miR-9 mimic or inhibitor on cisplatin sensitivity. miR-9 may be a potential biomarker for enhancing cisplatin sensitivity by regulating eIF5A2 in NSCLC cells.

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Citations

Mar 1, 2020·International Journal of Molecular Sciences·Julien GuindeElise Kaspi
May 20, 2020·PLoS Computational Biology·Chao KongYing-Cheng Lai
Mar 4, 2020·Molecular Cancer·Xinyi ZhangYongguang Tao
Aug 11, 2020·Computational and Structural Biotechnology Journal·Shicai LiuJinke Wang

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Methods Mentioned

BETA
PCR
protein assay
electrophoresis
transfection
flow cytometry

Software Mentioned

GraphPad
GraphPad Prism
TargetScan

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