MicroRNA-92a promotes tumor growth and suppresses immune function through activation of MAPK/ERK signaling pathway by inhibiting PTEN in mice bearing U14 cervical cancer.

Cancer Medicine
Zeng-Hui LiYan Wang

Abstract

Cervical cancer is known as the possible outcome of genital infection, while the molecular mechanisms of initiation, development, and metastasis of cervical cancer have not yet been fully elucidated. Our study aims to investigate the effects of microRNA-92a (miR-92a) on tumor growth and immune function by targeting PTEN via the MAPK/ERK signaling pathway in tumor-bearing mice. C57BL/6 female mice were used for tumor-bearing mouse models and their tumor and adjacent normal tissues were collected, and normal cervical tissues were obtained from normal mice. Serum levels of tumor necrosis factor-α (TNF-α) and soluble interleukin-2 receptor (sIL-2R) were detected by ELISA. The cells were divided into the normal, blank, negative control (NC), miR-92a mimic, miR-92a inhibitor, siRNA-PTEN, and miR-92a inhibitor + siRNA-PTEN groups. Dual-luciferase reporter assay was adopted to determine the relationship between PTEN and miR-92a. Expressions of miR-92a, PTEN, TNF-α, sIL-2R, ERK1, and ERK2 were tested by RT-qPCR and Western blotting. Cell proliferation was detected by cell count kit-8 (CCK-8); cell cycle and apoptosis were detected by flow cytometry. Compared with the normal cervical tissues and adjacent normal tissues, the cervical canc...Continue Reading

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Citations

Oct 12, 2018·Journal of Cellular Biochemistry·Xiang LiuMeiyun Zheng
Nov 22, 2019·Pathology Oncology Research : POR·Lei DingYanli Li
May 16, 2020·Cell Death & Disease·Kan ZhangLingli Luo
May 8, 2020·Oxidative Medicine and Cellular Longevity·Xiaoji CuiYing Liang
May 17, 2021·Pathology, Research and Practice·Kazem NejatiAmirAhmad Arabzadeh

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Methods Mentioned

BETA
ELISA
transfection
PCR
electrophoresis
Flow cytometry

Software Mentioned

TargetScan
SPSS

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