MicroRNA-9a-5p Alleviates Ischemia Injury After Focal Cerebral Ischemia of the Rat by Targeting ATG5-Mediated Autophagy

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Ning WangLihua Wang

Abstract

Previous studies have suggested that autophagy is activated in distinct cerebrovascular diseases, including stroke. However, the underlying regulatory mechanism of autophagy under stroke remained elusive. Accumulating evidence indicates that dysfunctions of microRNAs (miRNAs) are involved in the pathological process of stroke. Therefore, this study was taken to identify the effect of microRNA-9a-5p (miR-9a-5p) on autophagy in rats following stroke. The rat model of focal cerebral ischemia was established by middle cerebral artery occlusion (MCAO) surgery; The neurological outcomes were defined by neurological evaluation and infarct volume; The western blotting and immunofluorescence assays were used to detected the protein levels of microtubule-associated protein 1 light chain 3 (LC3) and autophagy related 5 (ATG5); The mRNA level of miR-9a-5p, LC3 and ATG5 were quantified by real-time RT-PCR; The luciferase activities of ATG5 and miR-9a-5p was detected by luciferase assay. We showed here that the level of miR-9a-5p was decreased in the ischemic region of rats after MCAO. Overexpression of miR-9a-5p by miR-9a-5p agomir reduced infarct volume and alleviated neurological deficit. Moreover, we found that autophagy was activated by...Continue Reading

Citations

Sep 27, 2018·Cell Death & Disease·Jianguo HuXiaojing Dong
Jul 6, 2019·Frontiers in Neuroscience·Audrey M ThiebautBenoit D Roussel
Jan 31, 2020·Neural Regeneration Research·Yun MoKang-Yong Liu
Aug 15, 2018·Neural Regeneration Research·Xian-Jun WangXiao-Yan Fu
Feb 3, 2021·Autophagy·Soudeh Ghafouri-FardMohammad Taheri
Nov 3, 2020·Frontiers in Cellular Neuroscience·Jie LiuYi Yang
Feb 1, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Yunus Akkoc, Devrim Gozuacik
Aug 13, 2021·Neural Regeneration Research·Ben Mead, Stanislav Tomarev
Sep 2, 2021·Neuropsychiatric Disease and Treatment·Xiaowang GuoZhijun Yong
Nov 7, 2021·Cellular and Molecular Life Sciences : CMLS·Rohan Gupta Pravir Kumar

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