MicroRNA and mRNA profiling in the idiopathic inflammatory myopathies.

BMC Rheumatology
Joanna E ParkesJanine A Lamb

Abstract

The idiopathic inflammatory myopathies (IIMs) are heterogeneous autoimmune conditions of skeletal muscle inflammation and weakness. MicroRNAs (miRNAs) are short, non-coding RNA which regulate gene expression of target mRNAs. The aim of this study was to profile miRNA and mRNA in IIM and identify miRNA-mRNA relationships which may be relevant to disease. mRNA and miRNA in whole blood samples from 7 polymyositis (PM), 7 dermatomyositis (DM), 5 inclusion body myositis and 5 non-myositis controls was profiled using next generation RNA sequencing. Gene ontology and pathway analyses were performed using GOseq and Ingenuity Pathway Analysis. Dysregulation of miRNAs and opposite dysregulation of predicted target mRNAs in IIM subgroups was validated using RTqPCR and investigated by transfecting human skeletal muscle cells with miRNA mimic. Analysis of differentially expressed genes showed that interferon signalling, and anti-viral response pathways were upregulated in PM and DM compared to controls. An anti-Jo1 autoantibody positive subset of PM and DM (n = 5) had more significant upregulation and predicted activation of interferon signalling and highlighted T-helper (Th1 and Th2) cell pathways. In miRNA profiling miR-96-5p was signific...Continue Reading

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Citations

Sep 9, 2021·Current Opinion in Rheumatology·Valérie LeclairIngrid E Lundberg

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Methods Mentioned

BETA
blood draw
chips
transfection
RNAseq
biopsies

Software Mentioned

Ingenuity Pathway Analysis ( IPA )
Ingenuity Pathway Analysis ( IPA
STAR
Trimmomatic
DESeq2
Canonical Pathways
Ingenuity Pathway Analysis ( IPA ) Pathways
MicroRNA Target Filter
Ingenuity Pathway Analysis ‘ Canonical Pathways ’
Venny

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