MicroRNA Contents in Matrix Vesicles Produced by Growth Plate Chondrocytes are Cell Maturation Dependent

Scientific Reports
Zhao LinB D Boyan

Abstract

Chondrocytes at different maturation states in the growth plate produce matrix vesicles (MVs), membrane organelles found in the extracellular matrix, with a wide range of contents, such as matrix processing enzymes and receptors for hormones. We have shown that MVs harvested from growth zone (GC) chondrocyte cultures contain abundant small RNAs, including miRNAs. Here, we determined whether RNA also exists in MVs produced by less mature resting zone (RC) chondrocytes and, if so, whether it differs from the RNA in MVs produced by GC cells. Our results showed that RNA, small RNA specifically, was present in RC-MVs, and it was well-protected from RNase by the phospholipid membrane. A group of miRNAs was enriched in RC-MVs compared RC-cells, suggesting that miRNAs are selectively packaged into MVs. High throughput array and RNA sequencing showed that ~39% miRNAs were differentially expressed between RC-MVs and GC-MVs. Individual RT-qPCR also confirmed that miR-122-5p and miR-150-5p were expressed at significantly higher levels in RC-MVs compared to GC-MVs. This study showed that growth plate chondrocytes at different differentiation stages produce different MVs with different miRNA contents, further supporting extracellular vesicle...Continue Reading

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Citations

Aug 14, 2019·Molecular Oncology·Carolini KaidOswaldo Keith Okamoto
Aug 2, 2020·Journal of Bone and Mineral Metabolism·Sushma BommanavarDowen Birkhed
Jan 18, 2019·Journal of Cellular Biochemistry·Chunrong ZhaoShiwu Dong
Dec 12, 2018·Frontiers in Cardiovascular Medicine·Yin TintutLinda L Demer
Jun 1, 2018·Scientific Reports·Abdullah Y MandourahDong L Barraclough
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Benjamin GantenbeinDevina Purmessur
Jun 15, 2020·Journal of Oral Biosciences·Yuji Yoshiko, Tomoko Minamizaki
Jan 9, 2020·Hormone Research in Pædiatrics·Francesca CirilloMaria Elisabeth Street

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Methods Mentioned

BETA
PCR
RNA-Seq

Software Mentioned

FASTQC
Seq
Nanosight
miARma
Bowtie2

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