microRNA-directed cleavage and translational repression of the copper chaperone for superoxide dismutase mRNA in Arabidopsis

The Plant Journal : for Cell and Molecular Biology
Linda BeauclairNicolas Bouché

Abstract

microRNA398 (miR398) is a conserved miRNA of plants that targets two of the three copper/zinc superoxide dismutases (SOD) of Arabidopsis (CSD1 and CSD2) by triggering cleavage or inhibiting translation of their mRNAs. We analysed the transcriptomes of mutants impaired in miR398 production, and found that the mRNAs encoding the copper chaperone for superoxide dismutase (CCS1), which delivers copper to CSD1 and CSD2 apoproteins in different cellular compartments, are undiscovered targets of miR398. We identified the cleavage site in CCS1 mRNAs by 5'-RACE PCR. We further show that both CCS1 protein and mRNA levels are tightly linked to the quantities of miR398, which are themselves dependent on the copper content in the medium. We generated transgenic plants carrying a CCS1 mRNA version resistant to cleavage by miR398, and demonstrated that both CCS1 mRNAs and proteins accumulate in these plants when miR398 is abundant and copper limiting. Moreover, we show that one of the ten ARGONAUTE proteins of Arabidopsis (AGO10) is involved in miR398-directed translational inhibition of CCS1 mRNAs, as CCS1 protein, but not CCS1 mRNAs accumulates in ago10 (zll) mutants. Thus, miR398 mediates the cleavage and translational inhibition of mRNAs ...Continue Reading

References

Sep 20, 1997·The Journal of Biological Chemistry·V C CulottaJ D Gitlin
Nov 7, 1999·Molecular Plant-microbe Interactions : MPMI·D J KliebensteinJ L Dangl
Aug 31, 2002·Cell·Matthew W RhoadesDavid P Bartel
Jan 10, 2003·Nucleic Acids Research·Mark L CroweMartin Trick
Jun 18, 2004·Molecular Cell·Matthew W Jones-Rhoades, David P Bartel
Jul 20, 2004·The Plant Cell·Ramanjulu Sunkar, Jian-Kang Zhu
Jul 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric BonnetYves Van de Peer
Sep 4, 2004·Genome Biology·Xiu-Jie WangTerry Gaasterland
Sep 24, 2004·RNA·Marc RehmsmeierRobert Giegerich
Apr 6, 2005·Developmental Cell·Rebecca SchwabDetlef Weigel
Apr 26, 2005·FEBS Letters·Salah E Abdel-GhanyMarinus Pilon
Apr 27, 2005·Cell·Edwards AllenJames C Carrington
Jul 18, 2006·Nucleic Acids Research·Jan Krüger, Marc Rehmsmeier
Dec 22, 2006·Genes & Development·Ramya RajagopalanDavid P Bartel
Feb 1, 2007·Plant, Cell & Environment·Tzyy-Jen Chiou
Apr 5, 2007·The Journal of Biological Chemistry·Hiroaki YamasakiMarinus Pilon
Oct 18, 2007·Nucleic Acids Research·Séverine GagnotVéronique Brunaud
Apr 9, 2008·Plant Molecular Biology·Diana V Dugas, Bonnie Bartel
May 17, 2008·Science·Peter BrodersenOlivier Voinnet
May 27, 2008·Trends in Plant Science·Allison C Mallory, Nicolas Bouché
Jun 11, 2008·Nature Biotechnology·Marcelo A GermanPamela J Green
Oct 25, 2008·The Journal of Biological Chemistry·Kim KirbyJohn P Phillips
Jan 16, 2009·Nature Reviews. Molecular Cell Biology·Peter Brodersen, Olivier Voinnet
Jan 17, 2009·Planta·Guru JagadeeswaranRamanjulu Sunkar
Feb 26, 2009·Cell·Olivier Voinnet
May 7, 2009·Nucleic Acids Research·Leonardo AlvesThomas Merkle
Jun 18, 2009·The Plant Cell·Elodie LanetChristophe Robaglia
Dec 17, 2009·Planta·Inês TrindadeDulce Metelo Dos Santos

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Citations

Mar 5, 2011·Plant Molecular Biology·Tzahi Arazi
Oct 15, 2011·Plant Molecular Biology·Matthew W Jones-Rhoades
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Li YangR Scott Poethig
Nov 28, 2013·Genome Biology and Evolution·Lei GongJonathan F Wendel
Aug 24, 2012·Journal of Experimental Botany·Sikander Pal ChoudharyLam-Son Phan Tran
Jul 25, 2013·The Plant Cell·Kestrel Rogers, Xuemei Chen
Aug 29, 2013·Biochemistry. Biokhimii︠a︡·P P Pashkovskiy, S S Ryazansky
Dec 31, 2011·BMC Genomics·Ashwani Jha, Ravi Shankar
Apr 2, 2013·PloS One·Mariana Carnavale BottinoPaulo Cavalcanti Gomes Ferreira
Aug 3, 2013·The Arabidopsis Book·Bijoyita Roy, Albrecht G von Arnim
Jan 15, 2014·Plant Cell Reports·Huyi HeMinghua Gu
Feb 25, 2014·Nucleic Acids Research·Cécile BrousseNicolas Bouché
May 30, 2014·Applied Biochemistry and Biotechnology·Rajesh Kumar
Jan 22, 2013·Annual Review of Plant Biology·Michael J Axtell
Sep 23, 2014·Trends in Plant Science·Junyan LiAnthony A Millar
Aug 12, 2014·Nucleic Acids Research·Tino KösterDorothee Staiger
Sep 4, 2014·Journal of Experimental Botany·Katarzyna KruszkaZofia Szweykowska-Kulinska
Jan 17, 2016·Gene Expression Patterns : GEP·Nada Babiker HamzaNeeti Sanan-Mishra
Jun 1, 2012·Journal of Plant Physiology·Katarzyna KruszkaFranck Vazquez
Mar 1, 2012·Trends in Plant Science·Ramanjulu SunkarGuru Jagadeeswaran
Mar 13, 2012·Plant Science : an International Journal of Experimental Plant Biology·Ligang Ren, Guiliang Tang
Dec 18, 2015·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Ying-Tao ZhaoYan-Tao Jia
Aug 31, 2012·The New Phytologist·Jeng-Shane LinShih-Tong Jeng
Mar 14, 2013·The Plant Journal : for Cell and Molecular Biology·Qingmei GuanJianhua Zhu
Apr 19, 2011·Physiologia Plantarum·Cheng ZhuHaili Liu
Oct 22, 2011·Journal of Integrative Plant Biology·Shanfa LuVincent L Chiang
Oct 30, 2013·The Plant Journal : for Cell and Molecular Biology·Guru JagadeeswaranRamanjulu Sunkar
Mar 20, 2014·Physiologia Plantarum·Xin SunJinggui Fang

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