MicroRNA Dysregulation in Pulmonary Arteries from Chronic Obstructive Pulmonary Disease. Relationships with Vascular Remodeling

American Journal of Respiratory Cell and Molecular Biology
Melina M MusriJoan A Barberà

Abstract

Pulmonary vascular remodeling is an angiogenic-related process involving changes in smooth muscle cell (SMC) homeostasis, which is frequently observed in chronic obstructive pulmonary disease (COPD). MicroRNAs (miRNAs) are small, noncoding RNAs that regulate mRNA expression levels of many genes, leading to the manifestation of cell identity and specific cellular phenotypes. Here, we evaluate the miRNA expression profiles of pulmonary arteries (PAs) of patients with COPD and its relationship with the regulation of SMC phenotypic change. miRNA expression profiles from PAs of 12 patients with COPD, 9 smokers with normal lung function (SK), and 7 nonsmokers (NS) were analyzed using TaqMan Low-Density Arrays. In patients with COPD, expression levels of miR-98, miR-139-5p, miR-146b-5p, and miR-451 were upregulated, as compared with NS. In contrast, miR-197, miR-204, miR-485-3p, and miR-627 were downregulated. miRNA-197 expression correlated with both airflow obstruction and PA intimal enlargement. In an in vitro model of SMC differentiation, miR-197 expression was associated with an SMC contractile phenotype. miR-197 inhibition blocked the acquisition of contractile markers in SMCs and promoted a proliferative/migratory phenotype mea...Continue Reading

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Citations

Nov 27, 2019·Journal of Cellular and Molecular Medicine·Zhuona XiXiaolan Zhong
Mar 12, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sangwoon ChungJohn W Christman
Sep 29, 2020·International Journal of Chronic Obstructive Pulmonary Disease·Mengchan ZhuMeiling Jin
Jun 23, 2020·Computational and Mathematical Methods in Medicine·Ran LiRuixue Xia
Apr 9, 2019·American Journal of Respiratory and Critical Care Medicine·Wassim W LabakiSurya P Bhatt
Oct 22, 2020·The Journal of Biological Chemistry·Cherry WongtrakoolBum-Yong Kang
Jan 18, 2021·The Journal of Biological Chemistry·Cherry WongtrakoolBum-Yong Kang

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Methods Mentioned

BETA
antisense oligonucleotides
transfection
PCR

Software Mentioned

limma
TargetScan

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