MicroRNA expression profile of mature dendritic cell in chronic rhinosinusitis

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Zu-Xia MaGuo-Hua Hu

Abstract

Chronic rhinosinusitis (CRS), which includes CRS without nasal polyposis (CRSsNP) and with nasal polyposis (CRSwNP), shows imbalance of helper T cells (Th) and regulatory T cells (Treg). The balance of Th and Treg cells is orchestrated by dendritic cells (DCs). Recent studies show functions of DCs can be regulated by microRNAs (miRNAs or miRs). This study is aimed to investigate miRNAs expression profiles of peripheral blood DCs in CRS. Peripheral blood samples of 30 patients with CRS and 7 patients with nasal septum deviation alone were collected. CD14(+) monocytes were isolated from these samples and differentiated into dendritic cells (DCs). Small RNAs were extracted from mature DCs and reversely transcribed into cDNA by Mir-XTM miRNA First-Strand synthesis method. MiRNA microarrays were used for miRNA expression analysis. Microarray results were validated by real-time PCR performed on five top list target genes. MiRNA microarrays showed that DCs from different types of patients have different sets of differential expressed miRNAs when comparing with Controls; they also share 31 commonly changed miRNAs among all three groups of CRS patients. Of these 31 miRNAs, 5 miRNAs were up-regulated and 25 miRNAs were down-regulated in ...Continue Reading

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Citations

Jul 19, 2016·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Stefano PirròCarla Montesano
Nov 20, 2016·Journal of the Science of Food and Agriculture·Ying-Hui HouChao-Ling Wei
May 28, 2019·Immunological Investigations·Krzysztof SpecjalskiEwa Jassem
Oct 16, 2019·Experimental and Therapeutic Medicine·Linge LiRongfu Huang
May 29, 2018·Cellular and Molecular Life Sciences : CMLS·Tom A P DriedonksEsther N M Nolte-'t Hoen
Nov 22, 2016·Current Opinion in Allergy and Clinical Immunology·Claudina Perez-Novo, Rogério Pezato
Feb 10, 2020·The Journal of Allergy and Clinical Immunology·Asawari KordeShervin S Takyar

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