MicroRNA expression profiles of cancer stem cells in head and neck squamous cell carcinoma

International Journal of Oncology
Kazuya YataNoboru Yamanaka

Abstract

Increasing evidence indicates that cancer stem cells have essential roles in tumor initiation, progression, metastasis and resistance to chemo-radiation. Recent research has pointed out biological importance of microRNAs in cancer stem cell dysregulation. Total number of mature microRNAs in human genome increased to more than 2,500 with the recent up-date of the database. However, currently no information is available regarding microRNA expression profiles of cancer stem cells in head and neck squamous cell carcinoma (HNSCC). Increased ALDH1 activity has been demonstrated as a reliable marker for isolation of cancer stem cells. Therefore, we evaluated the microRNA expression profile of ALDH1-high subpopulations in the HNSCC cell lines UTSCC-9 and UTSCC-90. Initially, we examined cancer stem cell properties of ALDH1-high subpopulations in both cell lines. We analyzed expression of stemness markers, sphere formation capacity and xenograft transplantation into NOD/SCID mice. Our findings validated that ALDH1-high subpopulations showed significantly increased tumor-initiating ability. Furthermore, we investigated the microRNA expression profile of HNSCC stem cells using microRNA array and confirmed the results by quantitative real-...Continue Reading

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Citations

Jul 30, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Chiranjib ChakrabortySrijit Das
Mar 26, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shuangzhan LiLei Zhang
May 1, 2021·Cancers·Melysa FitrianaTsai-Tsen Liao

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Methods Mentioned

BETA
fluorescence-activated cell sorting
FACS
PCR
flow cytometry
xenograft

Software Mentioned

TargetScan
Microarray Data Analysis Tool
GraphPad Prism
GraphPad

Related Concepts

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