MicroRNA expression profiling in PBMCs of Indian water Buffalo (Bubalus bubalis ) infected with Brucella and Johne's disease

ExRNA
Jasdeep SinghChandra Sekhar Mukhopadhyay

Abstract

MicroRNAs play key roles in host-pathogen-interactions and disease pathogenesis. Our aim was to characterize the differentially expressed miRNAs in the blood cells of diseased (Brucellosis-positive, Johne's disease-positive) and healthy- water buffaloes. The pooled small-RNA samples of each group were sequenced on Ion Torrent Personal Genome Machine (PGM) sequencer and the data were analyzed for differential expression. Here we identified 274 known miRNAs with bovine homologs and 36 novel mature-star miRNAs from the sequnces of small RNA libraries. Overall 195 miRNAs were common to all the three groups. Certain miRNAs such as bta-miR-21-5p, -26a, -29a/b, -30d - 103, - 140, - 150, - 191, - 374, - 1434-5p,-1260b, - 2484 and let-7 members were abundantly expressed in diseased groups. Bta-miR-1434-5p, - 188, -200c were up-regulated (> 1.5 folds) while bta-miR-27a-5p, -34b and -2285x were down-regulated (> 100 folds) in Brucellosis group. In Johne's Disease group, only 3 miRNAs (bta-miR-1434-5p, - 2340 and - 2484) were up-regulated (> 1.5 folds). The functional classification of miRNA target genes into gene ontology (GO) terms indicated their involvement in innate immunity and cellular process of disease pathogenesis. Expression pro...Continue Reading

References

Dec 6, 1969·The Veterinary Record·W J MorganG A Cullen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 5, 2003·Comparative Immunology, Microbiology and Infectious Diseases·J HostetterN Cheville
Apr 5, 2005·Nature Genetics·Azra KrekNikolaus Rajewsky
Dec 31, 2008·BMC Bioinformatics·Peter Langfelder, Steve Horvath
Feb 28, 2009·The Veterinary Journal·Peter A Windsor, Richard J Whittington
Sep 19, 2009·Nature Reviews. Genetics·Carlo M Croce
Jun 11, 2010·European Heart Journal·Yuri D'AlessandraMaurizio C Capogrossi
Jan 21, 2011·Nature·Eric M Small, Eric N Olson
Mar 10, 2011·Physiological Genomics·Nai-Kui Liu, Xiao-Ming Xu
Jun 16, 2011·International Journal of Molecular Sciences·De-Cai YuYi-Tao Ding
Oct 15, 2011·Journal of Clinical Microbiology·Yurong FuFuliang Xu
Nov 5, 2011·Nucleic Acids Research·Vera HuangLong-Cheng Li
Nov 11, 2011·Wiley Interdisciplinary Reviews. RNA·Shobha Vasudevan
Jun 6, 2012·RNA Biology·Ana EulalioJörg Vogel
Aug 21, 2012·International Journal of Biological Sciences·Ke ZhengZheng-Fei Liu
Sep 5, 2012·Trends in Biochemical Sciences·Andrey TurchinovichBarbara Burwinkel
Sep 11, 2012·Journal of Animal Science and Biotechnology·Kang KangDeming Gou
Dec 12, 2012·Experimental and Therapeutic Medicine·Weiguo SuiYong Dai
Apr 6, 2013·Cell Biochemistry and Biophysics·Amal A Abd-El-FattahMariam Lotfy Aboulftouh
Jul 23, 2013·Nature Protocols·Huaiyu MiPaul D Thomas
Nov 28, 2013·PloS One·Paolo MiottoDaniela M Cirillo
Aug 1, 2014·Frontiers in Genetics·Manali D Mehta, Philip T Liu
Nov 8, 2014·Nucleic Acids Research·Nathan Wong, Xiaowei Wang
Aug 13, 2015·ELife·Vikram AgarwalDavid P Bartel
Feb 27, 2017·Preventive Veterinary Medicine·R L SmithY T Gröhn

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRR3383406

Methods Mentioned

BETA
PCR
ELISA
density-based gradient centrifugation
RNA-Seq
chips

Software Mentioned

Venn Diagrams
WGCNA package
PANTHER Classification System
SDS
2100
R
PicTar
TargetScan

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved