MicroRNA Expression Profiling in the Prefrontal Cortex: Putative Mechanisms for the Cognitive Effects of Adolescent High Fat Feeding

Scientific Reports
Marie A LabouesseJ Richetto

Abstract

The medial prefrontal cortex (mPFC), master regulator of higher-order cognitive functions, is the only brain region that matures until late adolescence. During this period, the mPFC is sensitive to stressful events or suboptimal nutrition. For instance, high-fat diet (HFD) feeding during adolescence markedly impairs prefrontal-dependent cognition. It also provokes multiple changes at the cellular and synaptic scales within the mPFC, suggesting that major transcriptional events are elicited by HFD during this maturational period. The nature of this transcriptional reprogramming remains unknown, but may include epigenetic processes, in particular microRNAs, known to directly regulate synaptic functions. We used high-throughput screening in the adolescent mouse mPFC and identified 38 microRNAs differentially regulated by HFD, in particular mir-30e-5p. We used a luciferase assay to confirm the functional effect of mir-30e-5p on a chosen target: Ephrin-A3. Using global pathway analyses of predicted microRNA targets, we identified biological pathways putatively affected by HFD. Axon guidance was the top-1 pathway, validated by identifying gene expression changes of axon guidance molecules following HFD. Our findings delineate major m...Continue Reading

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Citations

Jun 18, 2020·Nutrients·Saivageethi NuthikattuAmparo C Villablanca
Aug 28, 2019·Neurobiology of Disease·Joy Jones BuieCatrina Sims-Robinson

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Datasets Mentioned

BETA
GSE105794

Methods Mentioned

BETA
PCR
transfection

Software Mentioned

StatView
TargetScan
IPA
miRWalk
Affymetrix
TargetScanMouse
DIANA TOOLS
Ingenuity Pathway Analysis ( IPA )
Partek Genomics Suite
DIANA Pathway

Related Concepts

Related Feeds

Axon Guidance

Axon guidance is a complex neural developmental field that investigates mechanisms through which neurons send out axons to reach its target. Here is the latest research in this domain.

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