MicroRNA Expression Profiling on Paired Primary and Lymph Node Metastatic Breast Cancer Revealed Distinct microRNA Profile Associated With LNM

Frontiers in Oncology
Ramesh ElangoNehad M Alajez

Abstract

Breast cancer (BC) is the foremost cause of cancer-related deaths in women. BC patients are oftentimes presented with lymph node metastasis (LNM), which increases their risk of recurrence. Compelling data have recently implicated microRNAs in promoting BC metastasis. Therefore, the identification of microRNA (miRNA)-based molecular signature associated with LNM could provide an opportunity for a more personalized treatment for BC patients with high risk of LNM. In current study, we performed comprehensive miRNA profiling in matched primary breast and LNM and identified 40 miRNAs, which were differentially expressed in LNM compared to primary tumors. The expression of 14 miRNAs (Up: hsa-miR-155-5p, hsa-miR-150-5p, hsa-miR-146a-5p, hsa-miR-142-5p and down: hsa-miR-200a-3p, hsa-miR-200b-3p, hsa-miR-200c-3p, hsa-miR-205-5p, hsa-miR-210-3p, hsa-miR-214-3p, hsa-miR-141-3p, hsa-miR-127-3p, hsa-miR-125a-5p, and hsa-let-7c-5p) was subsequently validated in a second cohort of 32 breast and 32 matched LNM tumor tissues. Mechanistically, forced expression of hsa-miR-205-5p, or hsa-miR-214-3p epigenetically inhibited MDA-MB-231 cell proliferation, colony formation, and cell migration. Global gene expression profiling on MDA-MB-231 cells ove...Continue Reading

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Citations

Mar 6, 2021·Molecular Therapy. Methods & Clinical Development·Ramesh ElangoNehad M Alajez
Jul 31, 2021·Scientific Reports·Radhakrishnan Vishnubalaji, Nehad M Alajez
Aug 8, 2021·International Journal of Molecular Sciences·Matthew G DaveyMichael J Kerin

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Datasets Mentioned

BETA
GSE148848

Methods Mentioned

BETA
chip
Low
PCR
transfection

Software Mentioned

TargetScan
GraphPad Prism
CLC genomics workbench
SRA
GraphPad
GeneSpring

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