MicroRNA expression signature of castration-resistant prostate cancer: the microRNA-221/222 cluster functions as a tumour suppressor and disease progression marker

British Journal of Cancer
Yusuke GotoNaohiko Seki

Abstract

Our present study of the microRNA (miRNA) expression signature in castration-resistant prostate cancer (CRPC) revealed that the clustered miRNAs microRNA-221 (miR-221) and microRNA-222 (miR-222) are significantly downregulated in cancer tissues. The aim of this study was to investigate the functional roles of miR-221 and miR-222 in prostate cancer (PCa) cells. A CRPC miRNA signature was constructed by PCR-based array methods. Functional studies of differentially expressed miRNAs were analysed using PCa cells. The association between miRNA expression and overall survival was estimated by the Kaplan-Meier method. In silico database and genome-wide gene expression analyses were performed to identify molecular targets regulated by the miR-221/222 cluster. miR-221 and miR-222 were significantly downregulated in PCa and CRPC specimens. Kaplan-Meier survival curves showed that low expression of miR-222 predicted a short duration of progression to CRPC. Restoration of miR-221 or miR-222 in cancer cells revealed that both miRNAs significantly inhibited cancer cell migration and invasion. Ecm29 was directly regulated by the miR-221/222 cluster in PCa cells. Loss of the tumour-suppressive miR-221/222 cluster enhanced migration and invasio...Continue Reading

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Citations

Feb 20, 2016·International Journal of Molecular Sciences·Shinya TakigawaKazunori Hamamura
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Datasets Mentioned

BETA
GSE29079
GSE56243
GSE3167

Methods Mentioned

BETA
biopsy
PCR
transfection
biopsies
antisense oligonucleotides

Software Mentioned

TargetScan
GeneSpring GX
Stat Mate

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