MicroRNA Expression Varies according to Glucose Tolerance, Measurement Platform, and Biological Source

BioMed Research International
S DiasC Pheiffer

Abstract

Dysregulated microRNA (miRNA) expression is observed during type 2 diabetes (T2D), although the consistency of miRNA expression across measurement platform and biological source is uncertain. Here we report miRNA profiling in the whole blood and serum of South African women with different levels of glucose tolerance, using next generation sequencing (NGS) and quantitative real time PCR (qRT-PCR). Whole blood-derived miRNAs from women with newly diagnosed T2D (n= 4), impaired glucose tolerance (IGT) (n= 4), and normal glucose tolerance (NGT) (n= 4) were subjected to NGS, whereafter transcript levels of selected miRNAs were quantified in the whole blood and serum of these women using qRT-PCR. Of the five significantly differentially expressed miRNAs identified by NGS, only the directional increase of miR-27b in women with IGT compared to NGT was confirmed in whole blood and serum, using qRT-PCR. Functional enrichment of miR-27b gene targets identified biological pathways associated with glucose transport and insulin regulation. In conclusion, this study showed poor correlation in miRNA expression profiled using NGS and qRT-PCR and in whole blood and serum. The consistent increased expression of miR-27b in women with IGT compared ...Continue Reading

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Citations

Sep 24, 2019·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Timothy J O'BrienRafael V Davalos
Oct 21, 2017·Journal of Biomaterials Science. Polymer Edition·Elbay MalikmammadovNesrin Hasirci
Sep 29, 2018·International Journal of Molecular Sciences·Stephanie DiasSumaiya Adam
Mar 21, 2018·Molecular Diagnosis & Therapy·Carmen PheifferSumaiya Adam
May 22, 2019·Molecular Diagnosis & Therapy·Carmen PheifferSumaiya Adam
Jan 21, 2021·American Journal of Physiology. Gastrointestinal and Liver Physiology·Alexandre VillardRamaroson Andriantsitohaina
Jan 6, 2022·Archives of Physiology and Biochemistry·Vishwadeep ShelkeAnil Bhanudas Gaikwad

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Methods Mentioned

BETA
electrophoresis
PCR
Illumina sequencing

Software Mentioned

Microcosm
TargetScan
GraphPad Prism
Novoalign
Line Basecaller OLB
GraphPad
Normfinder
TopGO
Off
Arraystar

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