microRNA in Extracellular Vesicles Released by Damaged Podocytes Promote Apoptosis of Renal Tubular Epithelial Cells.

Cells
Jin Seok JeonSoon Hyo Kwon

Abstract

Tubular injury and fibrosis are associated with progressive kidney dysfunction in advanced glomerular disease. Glomerulotubular crosstalk is thought to contribute to tubular injury. microRNAs (miRNAs) in extracellular vesicles (EVs) can modulate distant cells. We hypothesized that miRNAs in EVs derived from injured podocytes lead to tubular epithelial cell damage. As proof of this concept, tubular epithelial (HK2) cells were cultured with exosomes from puromycin-treated or healthy human podocytes, and damage was assessed. Sequencing analysis revealed the miRNA repertoire of podocyte EVs. RNA sequencing identified 63 upregulated miRNAs in EVs from puromycin-treated podocytes. Among them, five miRNAs (miR-149, -424, -542, -582, and -874) were selected as candidates for inducing tubular apoptosis according to a literature-based search. To validate the effect of the miRNAs, HK2 cells were treated with miRNA mimics. EVs from injured podocytes induced apoptosis and p38 phosphorylation of HK2 cells. The miRNA-424 and 149 mimics led to apoptosis of HK2 cells. These results show that miRNAs in EVs from injured podocytes lead to damage to tubular epithelial cells, which may contribute to the development of tubular injury in glomerular di...Continue Reading

References

Jul 21, 1998·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·D C Cattran, P Rao
Sep 12, 1998·Kidney International·W KrizK V Lemley
May 16, 2002·Microscopy Research and Technique·Wilhelm Kriz
Dec 31, 2002·Physiological Reviews·Hermann PavenstädtMatthias Kretzler
Jan 9, 2004·Clinical and Experimental Nephrology·Katsuhiko Asanuma, Peter Mundel
Apr 13, 2004·Oncogene·Teiji Wada, Josef M Penninger
Oct 13, 2006·Journal of the American Society of Nephrology : JASN·Mauro AbbateGiuseppe Remuzzi
Jan 23, 2009·Journal of the American Society of Nephrology : JASN·Marie C HoganChristopher J Ward
Jun 2, 2009·Frontiers in Bioscience (Scholar Edition)·Frank Y MaDavid J Nikolic-Paterson
Oct 26, 2011·The Journal of Physiology·Jonathan M StreetJames W Dear
Nov 15, 2011·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Noriyuki SugiyamaTakahiko Yokoyama
Dec 6, 2011·Transplantation Reviews·Jose Luis Gorriz, Alberto Martinez-Castelao
Dec 14, 2012·American Journal of Physiology. Renal Physiology·Wilhelm KrizKevin V Lemley
Jan 1, 2013·Journal of the American Society of Nephrology : JASN·Fernanda T BorgesRaghu Kalluri
Jan 9, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Keita UchinoTakahiro Ochiya
Jan 15, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gang WangCheuk-Chun Szeto
Feb 1, 2013·American Journal of Physiology. Renal Physiology·Diping WangJoseph P Grande
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Jul 6, 2013·Cell Death & Disease·K WangP-F Li
Aug 16, 2013·American Journal of Physiology. Renal Physiology·Lin-Li LvBi-Cheng Liu
Sep 7, 2013·Journal of Extracellular Vesicles·Paulina Kucharzewska, Mattias Belting
Nov 14, 2013·PloS One·Federica BaruttaGabriella Gruden
Jul 19, 2014·Cancer Research·Annabell BischoffMonilola A Olayioye
Feb 17, 2015·European Journal of Clinical Investigation·Ali RamezaniDominic S Raj
Mar 10, 2015·Frontiers in Physiology·Arthur C-K Chung, Hui Y Lan
Feb 11, 2016·International Journal of Molecular Sciences·Nunzio IraciStefano Pluchino
Mar 21, 2016·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Soon Hyo KwonLilach O Lerman
Apr 30, 2016·Pharmacology & Therapeutics·Mira Pavkovic, Vishal S Vaidya
May 18, 2016·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Soon Hyo KwonLilach O Lerman
Nov 1, 2016·Nature Reviews. Nephrology·Rutger J MaasJack F Wetzels
Nov 2, 2016·PloS One·Kishore Kumar JellaAbdel A Alli
Feb 13, 2018·Journal of Extracellular Vesicles·Mercedes N MunkondaDylan Burger
Mar 20, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jianling WangQian Qian
Sep 27, 2018·Kidney Research and Clinical Practice·Seo-Yoon MinTae-Sun Ha

❮ Previous
Next ❯

Citations

Nov 24, 2020·Hypertension·Olga Martinez-ArroyoRaquel Cortes
Apr 20, 2021·Kidney Research and Clinical Practice·Sul A LeeTae-Hyun Yoo
Jul 3, 2021·International Journal of Molecular Sciences·Giulia CricrìFederica Collino
Oct 15, 2021·Cell Biology International·Mohammadreza ArdalanSepideh Zununi Vahed

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
transfection
smRNA-Seq
flow cytometry

Software Mentioned

SPSS
Bowtie
miRDeep2

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis