MicroRNA let-7b inhibits keratinocyte differentiation by targeting IL-6 mediated ERK signaling in psoriasis

Cell Communication and Signaling : CCS
Yan WuJulia Li Zhong

Abstract

The extensive involvement of microRNA (miRNA) in the pathophysiology of psoriasis is well documented. However, in order for this information to be useful in therapeutic manipulation of miRNA levels, it is essential that detailed functional mechanisms are elucidated. This study aimed to explore the effects of IL-6 targeting by let-7b and ERK1/2 mediated signaling on keratinocyte differentiation in psoriasis. Following imiquimod cream (IMQ) application to let-7bTG (keratinocyte-specific let-7b overexpression mouse) and control mice for 7 days, we analyzed erythema, scaling and thickening of skin. A dual luciferase reporter assay and bioinformatics was carried out to detect target gene of let-7b. Additionally, the differentiation markers were measured. Immunohistochemistry analyses demonstrate a relationship of let-7b with IL-6 and ERK signaling. we found let-7bTG inhibits acanthosis and reduces the disease severity by treatment with IMQ compared to wild-type mice. Further study illustrated that let-7b promotes differentiation of keratinocytes in vivo and in vitro. Using bioinformatics and reporter gene assays, we found that IL-6 is a target gene of let-7b. In psoriasis, high expression levels of IL-6 lead to increased acivation o...Continue Reading

References

May 30, 1998·The Journal of Biological Chemistry·E GredingerE Bengal
Feb 23, 2007·Nature·Michelle A LowesJames G Krueger
Feb 26, 2009·Cell·Richard W Carthew, Erik J Sontheimer
Aug 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wendy A GoodmanKevin D Cooper
Sep 30, 2010·Nature Reviews. Rheumatology·Erwin F WagnerErwin Tschachler
Oct 22, 2010·The Journal of Investigative Dermatology·Andrew JohnstonJames T Elder
Mar 14, 2013·Methods in Molecular Biology·Youliang Wang, Xiao Yang
Dec 12, 2013·Physiological Genomics·Jing Xia, Weixiong Zhang
Jun 10, 2014·Journal of Dermatological Science·Marianne B LøvendorfLone Skov
Jul 2, 2014·The British Journal of Dermatology·H VinterC Johansen
Jan 17, 2015·Molecular Immunology·Aditi ChandraRaghunath Chatterjee
Feb 28, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Hui XiongQing Guo
Jan 31, 2016·Biochemical and Biophysical Research Communications·Haigang ZhuZhiming Li
Apr 10, 2016·Experimental Dermatology·Kuo-Feng HuangSheau-Huei Chueh
Nov 29, 2016·Nature Communications·Paulina KuligBurkhard Becher
Feb 22, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Sankhiros BabapoorSoheil S Dadras
Mar 30, 2017·Open Medicine·Yuan Liu, Quanzhong Liu
May 12, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xiao-Ping HuBo Yu
May 14, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hui ShenZhibo Yang
Jun 20, 2017·Molecular Medicine Reports·Hongxin LangXining Pang
Oct 24, 2017·Journal of the European Academy of Dermatology and Venereology : JEADV·W-H Boehncke
May 16, 2018·Journal of Clinical Medicine·Sabine WächterPietro Di Fazio

❮ Previous
Next ❯

Citations

Nov 2, 2019·Archives of Dermatological Research·Kendice IpBenigno C Valdez
Jan 19, 2020·Journal of the European Academy of Dermatology and Venereology : JEADV·L PasqualiE Sonkoly
Oct 16, 2019·Frontiers in Immunology·Haijing WuQianjin Lu
Mar 24, 2021·Experimental Dermatology·Chang ZengJohann E Gudjonsson

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
antisense oligonucleotides
Assay
transgenic
PCR
transfection

Software Mentioned

miRanda
TargetScan

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.