MicroRNA mir-16 is anti-proliferative in enterocytes and exhibits diurnal rhythmicity in intestinal crypts.

Experimental Cell Research
Anita BalakrishnanAli Tavakkolizadeh

Abstract

The intestine exhibits profound diurnal rhythms in function and morphology, in part due to changes in enterocyte proliferation. The regulatory mechanisms behind these rhythms remain largely unknown. We hypothesized that microRNAs are involved in mediating these rhythms, and studied the role of microRNAs specifically in modulating intestinal proliferation. Diurnal rhythmicity of microRNAs in rat jejunum was analyzed by microarrays and validated by qPCR. Temporal expression of diurnally rhythmic mir-16 was further quantified in intestinal crypts, villi, and smooth muscle using laser capture microdissection and qPCR. Morphological changes in rat jejunum were assessed by histology and proliferation by immunostaining for bromodeoxyuridine. In IEC-6 cells stably overexpressing mir-16, proliferation was assessed by cell counting and MTS assay, cell cycle progression and apoptosis by flow cytometry, and cell cycle gene expression by qPCR and immunoblotting. mir-16 peaked 6 hours after light onset (HALO 6) with diurnal changes restricted to crypts. Crypt depth and villus height peaked at HALO 13-14 in antiphase to mir-16. Overexpression of mir-16 in IEC-6 cells suppressed specific G1/S regulators (cyclins D1-3, cyclin E1 and cyclin-depe...Continue Reading

References

Jan 1, 1991·Annals of the New York Academy of Sciences·L E SchevingR J Feuers
Dec 1, 1983·The American Journal of Anatomy·L E SchevingL A Scheving
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·K NakayamaD Y Loh
Oct 27, 2001·Science·M Lagos-QuintanaT Tuschl
Oct 27, 2001·Science·R C Lee, V Ambros
Nov 24, 2001·Neuron·A Claridge-ChangM W Young
Apr 12, 2002·World Journal of Surgery·Tien C KoR Daniel Beauchamp
Jun 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xiaoyue PanKen-Ichi Inui
Jan 28, 2004·Cell·Andrew W Murray
Apr 20, 2005·Oncogene·Amit DeshpandePhilip W Hinds
Sep 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Amelia CimminoCarlo M Croce
Nov 1, 2005·Nature·Jan KrützfeldtMarkus Stoffel
Nov 26, 2005·Science·Kyle Kai-How FarhDavid P Bartel
Apr 6, 2006·Cancer Causes & Control : CCC·Cheng Chi Lee
Jun 7, 2006·Current Biology : CB·Akhilesh B ReddyMichael H Hastings
Jan 24, 2007·Molecular and Cellular Biology·Peter S LinsleyLee Lim
Jun 8, 2007·Neuron·Hai-Ying M ChengKarl Obrietan
Jun 29, 2007·The Journal of Biological Chemistry·Shunbin XuDavid Valle
Aug 19, 2007·Cancer Research·Charles D JohnsonFrank J Slack
Aug 28, 2007·Cell Cycle·Jana K Gillies, Ian A J Lorimer
Sep 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi TazawaHitoshi Nakagama
Jan 24, 2008·Molecular and Cellular Biology·Irena IvanovskaMichele A Cleary
Feb 21, 2008·BMC Genomics·Maocheng YangIsaac Edery
May 2, 2008·International Journal of Cancer. Journal International Du Cancer·Lin XiaDaiming Fan
Aug 15, 2008·Nucleic Acids Research·Qin LiuXiaofei Zheng
Sep 13, 2008·Journal of Pharmacological Sciences·Adam T StearnsAli Tavakkolizadeh
Dec 17, 2008·Cancer Research·Christian J BraunMatthias Dobbelstein
Jun 3, 2009·Genes & Development·David GatfieldUeli Schibler
Sep 10, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Fumitaka TakeshitaTakahiro Ochiya

❮ Previous
Next ❯

Citations

Jan 8, 2013·Molecular Biotechnology·Maria SirakovMichelina Plateroti
Nov 13, 2012·Journal of Molecular Biology·Neel Mehta, Hai-Ying M Cheng
Feb 3, 2015·Blood Cells, Molecules & Diseases·Diego de Siqueira FigueredoTiago Gomes de Andrade
Jul 18, 2014·PloS One·Neeta AdhikariJennifer L Hall
Apr 23, 2013·Journal of Biological Rhythms·Diego de Siqueira FigueredoTiago Gomes de Andrade
Aug 6, 2016·PloS One·Niels H H HeegaardJan Fahrenkrug
Mar 16, 2012·Annals of Surgery·Anita BalakrishnanAli Tavakkolizadeh
Nov 5, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Aimee ParkerCarmen Pin
Jan 25, 2018·Annals of the Royal College of Surgeons of England·A Balakrishnan
Mar 7, 2013·Acta Physiologica·J Pácha, A Sumová
Jul 15, 2017·International Journal of Molecular Sciences·Rafael Chacolla-HuaringaSean-Patrick Scott
Aug 26, 2016·Endocrine Regulations·K VoglovaIveta Herichova
Jun 1, 2021·Chronobiology International·Geo Anna, Nisha N Kannan
Jul 3, 2021·Cancers·Paulína Pidíková, Iveta Herichová

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.