MicroRNA Sequencing Identifies a Serum MicroRNA Panel, Which Combined With Aspartate Aminotransferase to Platelet Ratio Index Can Detect and Monitor Liver Disease in Pediatric Cystic Fibrosis

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Diego A CalvopinaGrant A Ramm

Abstract

Cystic fibrosis (CF)-associated liver disease (CFLD) is a hepatobiliary complication of CF. Current diagnostic modalities rely on nonspecific assessments, whereas liver biopsy is the gold standard to assess severity of fibrosis. MicroRNAs (miRNAs) regulate liver disease pathogenesis and are proposed as diagnostic biomarkers. We investigated the combined use of serum miRNAs and aspartate aminotransferase (AST) to platelet ratio (APRI) to diagnose and assess CFLD severity. This was a cross-sectional cohort study of the circulatory miRNA signature of 124 children grouped by clinical, biochemical, and imaging assessments as follows: CFLD (n = 44), CF patients with no evidence of liver disease (CFnoLD; n = 40), and healthy controls (n = 40). Serum miRNAs were analyzed using miRNA sequencing (miRNA-Seq). Selected differentially expressed serum miRNA candidates were further validated by qRT-PCR and statistical analysis performed to evaluate utility to predict CFLD and fibrosis severity validated by liver biopsy, alone or in combination with APRI. Serum miR-122-5p, miR-365a-3p, and miR-34a-5p levels were elevated in CFLD compared to CFnoLD, whereas miR-142-3p and let-7g-5p were down-regulated in CFLD compared to CFnoLD. Logistic regres...Continue Reading

References

Oct 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A LindbladB Strandvik
Oct 7, 2004·Journal of Hepatology·Tamara N PereiraGrant A Ramm
Dec 8, 2004·Journal of Hepatology·Thierry LamireauFernando Alvarez
Mar 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·C KendziorskiM N Gould
Dec 22, 2006·The European Respiratory Journal·J A DodgeJ Wilsher
Mar 9, 2007·Bioinformatics·Wuyan ZhangJack C M Dekkers
Sep 29, 2007·Current Opinion in Pulmonary Medicine·Carla Colombo
Dec 31, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Grant A RammPeter J Lewindon
Apr 22, 2009·Current Opinion in Gastroenterology·Katie Moyer, William Balistreri
Feb 6, 2010·Pediatric Radiology·Renaud MentenPatrick Lebecque
Feb 12, 2010·Methods : a Companion to Methods in Enzymology·Evan M KrohMuneesh Tewari
Mar 24, 2010·International Journal of Cancer. Journal International Du Cancer·Fei-Fei LanHsiang-Fu Kung
Dec 15, 2010·Critical Reviews in Oncology/hematology·Glen ReidNico van Zandwijk
Jan 22, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Peter J LewindonGrant A Ramm
May 10, 2011·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·A C Malbrunot-WagnerA Dabadie
Jun 17, 2011·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Dominique DebrayCarla Colombo
Dec 26, 2012·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Thomas Flass, Michael R Narkewicz
Feb 26, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Matthew T KitsonStuart K Roberts
May 22, 2013·Nature Reviews. Gastroenterology & Hepatology·Gyongyi Szabo, Shashi Bala
Aug 22, 2013·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Steven N HartJean-Pierre Kocher
Jun 12, 2014·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Marion RowlandBilly Bourke
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Jan 13, 2015·International Journal of Hepatology·Thilde Nordmann WintherBirthe Hogh
Jan 28, 2015·Journal of Pediatric Gastroenterology and Nutrition·Naomi L CookGrant A Ramm
Aug 1, 2015·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Daniel H LeungGrant A Ramm
Oct 22, 2016·Journal of Pediatric Gastroenterology and Nutrition·Xiaofang PengPietro Invernizzi
Nov 2, 2016·International Journal of Molecular Sciences·Diego A CalvopinaGrant A Ramm
Jul 29, 2017·Journal of Pediatric Gastroenterology and Nutrition·Dominique DebrayHenkjan J Verkade
Nov 13, 2017·The American Journal of Pathology·Ying WanFanyin Meng
Jan 10, 2018·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Ruth H KeoghDiana Bilton

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Citations

Jul 16, 2019·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Diego A CalvopinaGrant A Ramm
Dec 23, 2020·Diagnostics·Fatima Domenica Elisa De PalmaMaria Chiara Maiuri
Jan 19, 2021·Archives of Biochemistry and Biophysics·Sujay PaulAashish Srivastava

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