MicroRNA signature associated with osteogenic lineage commitment.

Molecular Biology Reports
Behnaz BakhshandehNasser Ghaemi

Abstract

Cell-based approaches offer a potential therapeutic strategy for appropriate bone manufacturing. Capable of differentiating into multiple cell types especially osteoblasts spontaneously, unrestricted somatic stem cell (USSC) seems to be a suitable candidate. Recent studies have shown the involvement of microRNAs in several biological processes. miRNA microarray profiling was applied in order to identify the osteo-specific miRNA signature. Prior to this analysis, osteogenic commitment of osteoblasts was evaluated by measuring ALPase activity, biomineralization, specific staining and evaluation of some main osteogenic marker genes. To support our findings, various in silico explorations (for both putative targets and signaling pathways) and empirical analyses (miRNA transfections followed by qPCR of osteogenic indicators and ALPase activity measurement) were carried out. The function of GSK-3b inhibitor was also studied to investigate the role of WNT in osteogenesis. Transient modulation of multiple osteo-miRs (such as mir-199b, 1274a, 30b) with common targets (such as BMPR, TCFs, SMADs) as mediators of osteogenic pathways including cell-cell interactions, WNT and TGF-beta pathways, suggests a mechanism for rapid induction of the...Continue Reading

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Citations

May 2, 2012·Biotechnology Letters·Behnaz BakhshandehMasoud Soleimani
Oct 12, 2012·In Vitro Cellular & Developmental Biology. Animal·Maryam HafiziAmir Atashi
May 18, 2013·Molecular and Cellular Biochemistry·Najmeh RanjiKeyhan Azadmanesh
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Nov 21, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Xiao-Ming YuShi-Bi Lu
Jul 16, 2016·Stem Cells and Development·Claudine SeeligerMartijn van Griensven
Dec 23, 2017·Journal of Biomedical Materials Research. Part a·Mohammad Omid OftadehArash Khojasteh
Jan 30, 2016·In Vitro Cellular & Developmental Biology. Animal·Saghar HoseinzadehNosratollah Zarghami
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Sep 4, 2012·Journal of Genetics·Behnaz BakhshandehNasser Ghaemi
Dec 14, 2016·Journal of Cellular Biochemistry·Sandra StrassburgFilip Simunovic

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