MicroRNA signatures from multidrug‑resistant Mycobacterium tuberculosis

Molecular Medicine Reports
Na RenXingwang Li

Abstract

Tuberculosis (TB) infections, caused by multidrug‑resistant Mycobacterium tuberculosis (MDR MTB), remain a significant public health concern worldwide. The regulatory mechanisms underlying the emergence of MDR MTB strains remain to be fully elucidated, and further investigation is required in order to develop better strategies for TB control. The present study investigated the expression profile of microRNA (miRNA) in MTB strains, and examined the differences between sensitive MTB and MDR MTB using next generation sequencing (NGS) with Illumina Deep Sequencing technology to better understand the mechanisms of resistance in MDR MTB, A total of 5, 785 and 195, and 6, 290 and 595 qualified Illumina reads were obtained from two MDR MTB strains, and 6, 673 and 665, and 7, 210 and 217 qualified Illumina reads were obtained from two sensitive MTB strains. The overall de novo assembly of miRNA sequence data generated 62 and 62, and 95 and 112 miRNAs between the 18 and 30 bp long from sensitive MTB strains and MDR MTB strains, respectively. Comparative miRNA analysis revealed that 142 miRNAs were differentially expressed in the MDR MTB strain, compared with the sensitive MTB strain, of which 48 were upregulated and 94 were downregulated...Continue Reading

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Citations

Mar 22, 2016·Nature Reviews. Genetics·Sara A ByronDavid W Craig
May 31, 2018·Signal Transduction and Targeted Therapy·Xikun ZhouMin Wu
Dec 25, 2019·Infection and Immunity·Cinthya Ruiz-TagleMaría Elvira Balcells
Apr 14, 2018·Frontiers in Microbiology·Naveed SabirXiangmei Zhou
Jan 27, 2021·Drug Discovery Today·Amit Kumar SinghShripad A Patil
Jul 6, 2021·Parasitology·Sajad RashidiEugenio Antonio Carrera Silva

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Methods Mentioned

BETA
biopsy
PCR
electrophoresis
Assay
Illumina sequencing
DNA assay
in vitro transcription

Software Mentioned

Cutadapt
Target Rank
fastq
Bowtie
SPSS
fastx
INFERNAL
Mirdeep
cliper
Miranda

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