MicroRNA signatures in cardiac biopsies and detection of allograft rejection

The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation
Andrea Di FrancescoAnnalisa Angelini

Abstract

Identification of heart transplant (HTx) rejection currently relies on immunohistology and immunohistochemistry. We aimed to identify specific sets of microRNAs (miRNAs) to characterize acute cellular rejection (ACR), antibody-mediated rejection (pAMR), and mixed rejection (MR) in monitoring formalin-fixed paraffin-embedded (FFPE) endomyocardial biopsies (EMBs) in HTx patients. In this study we selected 33 adult HTx patients. For each, we chose the first positive EMB for study of each type of rejection. The next-generation sequencing (NGS) IonProton technique and reverse transcript quantitative polymerase chain reaction (RT-qPCR) analysis were performed on FFPE EMBs. Using logistic regression analysis we created unique miRNA signatures as predictive models of each rejection. In situ PCR was carried out on the same EMBs. We obtained >2,257 mature miRNAs from all the EMBs. The 3 types of rejection showed a different miRNA profile for each group. The logistic regression model formed by miRNAs 208a, 126-5p, and 135a-5p identified MR; that formed by miRNAs 27b-3p, 29b-3p, and 199a-3p identified ACR; and that formed by miRNAs 208a, 29b-3p, 135a-5p, and 144-3p identified pAMR. The expression of miRNAs on tissue, through in situ PCR, s...Continue Reading

Citations

May 8, 2019·Canadian Journal of Physiology and Pharmacology·Jody GroenendykMarek Michalak
Apr 23, 2019·Current Opinion in Organ Transplantation·Luciano PotenaOrnella Leone
May 16, 2019·Current Opinion in Organ Transplantation·Luciano PotenaOrnella Leone
Jan 6, 2021·Transplantation Reviews·Yeraz KhachatoorianMartin Cadeiras
Feb 20, 2021·Scientific Reports·Donato SantovitoFrancesco Cipollone
May 11, 2021·Frontiers in Cardiovascular Medicine·Haoyuan ZhengMingxiao Hou
Aug 4, 2021·Cardiovascular Research·Jordan S PoberGeorge Tellides
Aug 24, 2021·Circulation Journal : Official Journal of the Japanese Circulation Society·Lillian BenckJon Kobashigawa

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