MicroRNA‑142‑3p suppresses cell proliferation, invasion and epithelial‑to‑mesenchymal transition via RAC1‑ERK1/2 signaling in colorectal cancer.

Molecular Medicine Reports
Na XieYou-Sheng Huang

Abstract

Aberrant expression of microRNAs (miRNAs/miRs) is associated with the initiation and progression of colorectal cancer (CRC), but how they regulate colorectal tumorigenesis is still unknown. The present study was designed to investigate the expression profile of miRNAs in human CRC tissues, and to reveal the molecular mechanism of miRNA‑142‑3p in suppressing colon cancer cell proliferation. The expression of miRNA was examined using an Exiqon miRNA array. Bioinformatics was used to predict the target genes of differentially expressed miRNAs and to analyze their biological function in CRC. The effect of miR‑142‑3p in colon cancer cells was evaluated in vitro using cell proliferation, colony formation and Transwell assays. Dual‑luciferase reporter gene assays were performed to investigate the association between miR‑142‑3p and Rac family small GTPase 1 (RAC1). The effect of miR‑142‑3p regulation on colon cancer proliferation was assessed through western blotting and quantitative polymerase chain reaction analysis. Compared with their expression in adjacent non‑cancer mucosal tissues, 76 miRNAs were upregulated and 102 miRNAs were downregulated in CRC. One of the most significantly and differentially regulated miRNAs was miR‑142‑3p...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Dec 31, 2003·Cell·Benjamin P LewisChristopher B Burge
Jan 8, 2004·Genome Biology·Anton J EnrightDebora S Marks
Apr 5, 2005·Nature Genetics·Azra KrekNikolaus Rajewsky
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Oct 29, 2008·Genome Research·Robin C FriedmanDavid P Bartel
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jul 20, 2012·Nature·UNKNOWN Cancer Genome Atlas Network
Apr 27, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Wei-Wei ShenGuo-Hui Fu
Jan 31, 2015·International Journal of Molecular Sciences·Albert RenJun Yu
Sep 29, 2015·Iranian Journal of Cancer Prevention·Reza GhanbariReza Malekzadeh
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Aug 18, 2016·Small GTPases·Lan K NguyenAlex von Kriegsheim
Nov 25, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Amit ShresthaSaverio Bellusci
Dec 3, 2016·Nucleic Acids Research·Zhen YangAndrew E Teschendorff
Apr 5, 2018·Annals of Surgical Oncology·Martin R Weiser
Jun 10, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Kai ZhouYi-Fang Ping
Aug 2, 2018·Technology in Cancer Research & Treatment·Xiang GaoDong Hua
Aug 16, 2018·Nature Reviews. Molecular Cell Biology·Luca F R Gebert, Ian J MacRae
Mar 30, 2019·The Journal of International Medical Research·Wencang GaoSenquan Yu
Apr 7, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gayathri AnandappaNicola Valeri
Apr 28, 2019·Cells·Pradip DeNandini Dey
Jun 20, 2019·Cancer Management and Research·Dewei PengDongsheng Yan
Jun 27, 2019·Journal of Cellular Physiology·Behzad MansooriBehzad Baradaran
Jul 7, 2019·Journal of Clinical Medicine·Nikhil SahajpalRenu A Kowluru
Nov 2, 2019·Journal of Cellular Physiology·Tao XuShu-Kui Wang
Dec 13, 2019·Cell & Bioscience·Zahra KoveitypourKamran Ghaedi
Mar 18, 2020·Cancers·Larissa Kotelevets, Eric Chastre
Jun 18, 2020·Cells·Patrick Santos, Fausto Almeida
Jul 3, 2020·International Journal of Molecular Sciences·Paulína PidíkovaIveta Herichova
Aug 15, 2020·International Journal of Biological Sciences·Boxue HeXiang Wang
Sep 12, 2020·Cell Proliferation·Jiali LongWei Zhu

❮ Previous
Next ❯

Software Mentioned

Biovenn
ImageJ
DAVID
Targetscan
miRanda
Cytoscape
GenePix Pro
PicTar
SPSS Statistics

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.