MicroRNA218 inhibits glioma migration and invasion via inhibiting glioma-associated oncogene homolog 1 expression at N terminus

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Biao PengSu Hu

Abstract

Glioma is characterized by high invasion, migration and proliferation abilities. However, the molecular mechanism that triggers the development and recurrence of this tumor is also elusive. This study aims to investigate the biological function and molecular mechanism of microRNA218 in glioma. Human glioma samples were obtained and employed to investigate the correlation between microRNA218 and glioma pathological grading. Glioma cell viability was detected by the cell-counting kit-8 (CCK-8) cell counting assay. Transwell assay and wound-healing assay were employed to examine the migration and invasion of the glioma cells. The mRNA transcription and protein expression of glioma-associated oncogene homolog 1 (GLI1) were analyzed by quantitative RT-PCR and Western blot analysis, respectively. Southwestern blot assay was utilized to explore the possible interaction site of GLI1 and microRNA218. The results indicated that microRNA218 is significantly down-regulated in glioma samples and negatively correlated with the pathological grading. The cell viability was significantly decreased, and migration and invasion were significantly inhibited in microRNA218 treated cells, compared with un-treated cells. GLI1 was discovered acting as ...Continue Reading

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Citations

Apr 19, 2015·Journal of Experimental & Clinical Cancer Research : CR·Zhe ChengYouxin Zhou
Feb 20, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qiang RuanYan Ding
Jul 6, 2014·BioMed Research International·Guan SunJun Guo
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Aug 31, 2018·International Journal of Molecular Sciences·Maja SabolSonja Levanat
Feb 14, 2019·Cells·Paweł NiewiadomskiKatarzyna Chojnowska

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