MicroRNA‑665 suppresses the growth and migration of ovarian cancer cells by targeting HOXA10

Molecular Medicine Reports
Jinhui LiuWenJun Cheng

Abstract

Ovarian cancer is the most lethal gynecological cancer and its metastasis leads to a poor prognosis. The present study was designed to elucidate how microRNA (miR)‑665 regulates the proliferation and migration of ovarian tumor cells. Reverse transcription‑polymerase chain reaction (RT‑PCR) demonstrated that miR‑665 expression was decreased in ovarian cancer tissues. Increased expression of miR‑665 suppressed the growth and migration of ovarian cancer cells, whereas the downregulated expression of miR‑665 led to the opposite results. Bioinformatics tools identified homeobox A10 (HOXA10) as a target of miR‑665. Following miR‑665 overexpression, HOXA10 protein expression was significantly reduced. A dual luciferase assay revealed that miR‑665 bound to the 3'‑untranslated region of HOXA10. Immunohistochemistry and RT‑PCR revealed that the expression of HOXA10 was negatively correlated with the expression of miR‑665. It was concluded that miR‑665 targets HOXA10 and may act as a tumor‑suppressing gene in ovarian cancer. This pathway may be involved in the development and metastasis of ovarian cancer.

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Citations

Oct 1, 2020·International Journal of Molecular Sciences·Vu Hong Loan NguyenChun Peng
Aug 7, 2019·Cancers·Praveena IdaikkadarAgnieszka Michael
Aug 17, 2021·Analytical Cellular Pathology (Amsterdam)·Chenyang LiYesheng Wang
Sep 10, 2021·Journal of Experimental & Clinical Cancer Research : CR·Sipei NieWenjun Cheng

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Methods Mentioned

BETA
PCR
Protein Extraction
protein assay

Software Mentioned

SPSS
TargetScan7
PicTar
TargetScan

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