MicroRNA844-Guided Downregulation of Cytidinephosphate Diacylglycerol Synthase3 (CDS3) mRNA Affects the Response of Arabidopsis thaliana to Bacteria and Fungi

Molecular Plant-microbe Interactions : MPMI
Hwa Jung LeeHunseung Kang

Abstract

Despite the fact that a large number of miRNA sequences have been determined in diverse plant species, reports demonstrating the functional roles of miRNAs in the plant response to pathogens are severely limited. Here, Arabidopsis thaliana miRNA844 (miR844) was investigated for its functional role in the defense response to diverse pathogens. Transgenic Arabidopsis plants overexpressing miR844 (35S::miR844) displayed much more severe disease symptoms than the wild-type plants when challenged with the bacterium Pseudomonas syringae pv. tomato DC3000 or the fungus Botrytis cinerea. By contrast, a loss-of-function mir844 mutant showed an enhanced resistance against the pathogens. Although no cleavage was observed at the predicted cleavage site of the putative target mRNA, cytidinephosphate diacylglycerol synthase3 (CDS3), cleavage was observed at 6, 12, 21, or 52 bases upstream of the predicted cleavage site of CDS3 mRNA, and the level of CDS3 mRNA was downregulated by the overexpression of miR844, implying that miR844 influences CDS3 transcript level. To further confirm that the miR844-mediated defense response was due to the decrease in CDS3 mRNA level, the disease response of a CDS3 loss-of-function mutant was analyzed upon pat...Continue Reading

References

Nov 25, 1997·Biochimica Et Biophysica Acta·W Dowhan
Sep 29, 2000·Progress in Nucleic Acid Research and Molecular Biology·A Lykidis, S Jackowski
Aug 16, 2002·Current Opinion in Plant Biology·Ana M Laxalt, Teun Munnik
Oct 11, 2003·Plant Physiology·Isamu SakuraiHajime Wada
Apr 6, 2004·Plant Physiology·Ildikó DomonkosZoltán Gombos
Jun 18, 2004·Molecular Cell·Matthew W Jones-Rhoades, David P Bartel
Jan 13, 2005·Plant Physiology·Bindu NishalVenkatesan Sundaresan
Aug 5, 2005·Annual Review of Phytopathology·Jyoti Shah
Aug 27, 2005·Cell·Shveta BaggaAmy E Pasquinelli
Nov 24, 2005·Current Biology : CB·Hiroaki FujiiJian-Kang Zhu
Jan 3, 2006·The Plant Cell·Tzyy-Jen ChiouChun-Lin Su
Dec 22, 2006·Genes & Development·Ramya RajagopalanDavid P Bartel
Jul 20, 2007·The Plant Journal : for Cell and Molecular Biology·Shanfa LuVincent L Chiang
Nov 9, 2007·The Plant Journal : for Cell and Molecular Biology·Bikram Datt PantWolf-Rüdiger Scheible
Apr 9, 2008·Plant Physiology·Shu-I LinTzyy-Jen Chiou
Apr 16, 2008·The Plant Journal : for Cell and Molecular Biology·Jin Sun KimHunseung Kang
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Alex M MurphyDavid E Hanke
Aug 16, 2008·Science·Lionel NavarroOlivier Voinnet
Sep 20, 2008·The Plant Journal : for Cell and Molecular Biology·Cintia Goulart KawashimaTamas Dalmay
Jan 17, 2009·Planta·Guru JagadeeswaranRamanjulu Sunkar
Feb 26, 2009·Cell·Olivier Voinnet
Apr 30, 2009·Annual Review of Phytopathology·Aardra Kachroo, Pradeep Kachroo
Jun 13, 2009·Annual Review of Plant Biology·Virginia Ruiz-Ferrer, Olivier Voinnet
Jun 26, 2009·The Biochemical Journal·Hong-Wei XueYu Mei
Jul 18, 2009·Current Opinion in Plant Biology·Chellappan PadmanabhanHailing Jin
Jul 22, 2009·Biochimica Et Biophysica Acta·Riekelt H HoutkooperFrédéric M Vaz
Jun 11, 2010·Annual Review of Biochemistry·Marc Robert FabianWitold Filipowicz
Aug 7, 2010·Annual Review of Phytopathology·Surekha Katiyar-Agarwal, Hailing Jin

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Citations

Oct 11, 2017·Molecular Plant-microbe Interactions : MPMI·Raquel Salvador-GuiraoBlanca San Segundo
Apr 5, 2019·Plant Biotechnology Journal·Marcos Fernando BassoMaria Fatima Grossi-de-Sa
Nov 5, 2019·Frontiers in Plant Science·Stéphanie Jaubert-PossamaiBruno Favery
Feb 13, 2021·Biomolecules·Saquib WaheedZhangli Hu
Mar 30, 2021·Plant Biotechnology Journal·Sonia CampoBlanca San Segundo
May 27, 2021·Plant Physiology and Biochemistry : PPB·Kanika KhannaRenu Bhardwaj

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