MicroRNAome of porcine pre- and postnatal development.

PloS One
Mingzhou LiXuewei Li

Abstract

The domestic pig is of enormous agricultural significance and valuable models for many human diseases. Information concerning the pig microRNAome (miRNAome) has been long overdue and elucidation of this information will permit an atlas of microRNA (miRNA) regulation functions and networks to be constructed. Here we performed a comprehensive search for porcine miRNAs on ten small RNA sequencing libraries prepared from a mixture of tissues obtained during the entire pig lifetime, from the fetal period through adulthood. The sequencing results were analyzed using mammalian miRNAs, the precursor hairpins (pre-miRNAs) and the first release of the high-coverage porcine genome assembly (Sscrofa9, April 2009) and the available expressed sequence tag (EST) sequences. Our results extend the repertoire of pig miRNAome to 867 pre-miRNAs (623 with genomic coordinates) encoding for 1,004 miRNAs, of which 777 are unique. We preformed real-time quantitative PCR (q-PCR) experiments for selected 30 miRNAs in 47 tissue-specific samples and found agreement between the sequencing and q-PCR data. This broad survey provides detailed information about multiple variants of mature sequences, precursors, chromosomal organization, development-specific exp...Continue Reading

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Citations

Apr 5, 2014·PloS One·Paolo MartiniGerolamo Lanfranchi
Feb 7, 2014·BMC Genomics·Ting ChenYong-Liang Zhang
Feb 4, 2014·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Xiao MiXiaolin Liu
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Sep 20, 2011·International Journal of Biological Sciences·Mingzhou LiXuewei Li
Jan 3, 2012·International Journal of Biological Sciences·Qi ZhouXuewei Li
May 24, 2012·Nature Communications·Mingzhou LiRuiqiang Li
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Jan 8, 2015·Journal of Assisted Reproduction and Genetics·Zonggang LuoLing Wang
Jan 22, 2011·PloS One·Agnieszka PodolskaSusanna Cirera

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Datasets Mentioned

BETA
GSE17885

Methods Mentioned

BETA
Illumina sequencing
sequ-seqs
PCR

Software Mentioned

Illumina
UNAfold
lib
Genome Analyzer Pipeline
enoLOGOS
ACGT101
miR

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