MicroRNAs associated with tumour migration, invasion and angiogenic properties in A549 and SK-Lu1 human lung adenocarcinoma cells

Lung Cancer : Journal of the International Association for the Study of Lung Cancer
Chai San HoNoor Hasima

Abstract

Dysregulation in miRNA expression contributes towards the initiation and progression of metastasis by regulating multiple target genes. In this study, variations in miRNA expression profiles were investigated between high and low invasive NSCLC cell lines followed by identification of miRNAs with targets governing NSCLC's metastatic potential. Two NSCLC sub-cell lines possessing opposing migration and invasion properties were established using serial transwell invasion assays. Global miRNA expression profiles were obtained using microarray followed by RT-qPCR validation. Target prediction and pathway enrichment analyses were conducted on dysregulated miRNAs using DIANA-mirPath, DIANA-microT 4.0 and TargetScan 5.2 softwares. Metastatic effects of dysregulated miRNAs were evaluated using wound healing assay, invasion assay and HUVEC angiogenesis assay following transfection with mimics and inhibitors. A total of eleven differentially expressed miRNAs were revealed from microarray analyses, with four miRNAs validated through RT-qPCR. Three of these miRNAs were further selected for biological function validations, with only two modulating metastasis. A pathway model describing interactions between miRNAs and metastasis highlighted ...Continue Reading

References

Apr 21, 1997·The Journal of Cell Biology·R L KlemkeD A Cheresh
May 15, 1997·Nature·D I StruttM Mlodzik
Aug 19, 2000·Cellular and Molecular Life Sciences : CMLS·P Friedl, E B Bröcker
Dec 12, 2001·Circulation Research·B P Eliceiri
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 15, 2003·Nature Reviews. Cancer·John D Hood, David A Cheresh
Oct 2, 2004·Nature Reviews. Molecular Cell Biology·Wenjun Guo, Filippo G Giancotti
Nov 30, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Cheng-Long HuangMasaki Ueno
Dec 8, 2005·Journal of the National Cancer Institute·Haruhito AzumaShigeo Horie
Feb 24, 2006·The Journal of Cell Biology·Jean Paul ten KloosterPeter L Hordijk
May 26, 2006·Nature Reviews. Cancer·Rakesh KumarChristopher J Barnes
Nov 18, 2006·Cell·Gaorav P Gupta, Joan Massagué
Dec 26, 2006·The International Journal of Biochemistry & Cell Biology·Ora Bernard
May 2, 2007·Journal of Cellular Biochemistry·Ramesh A BhatPeter V N Bodine
Sep 13, 2007·International Journal of Cancer. Journal International Du Cancer·Suvi-Katri Leivonen, Veli-Matti Kähäri
Jan 15, 2008·Nature Cell Biology·Qihong HuangReuven Agami
Jul 8, 2008·Biochemical and Biophysical Research Communications·M CrawfordS P Nana-Sinkam
Sep 18, 2008·Angiogenesis·Pasquale CironeCraig M Crews
May 14, 2009·Bioinformatics·G L PapadopoulosA G Hatzigeorgiou
Aug 13, 2009·Molecular Cancer Therapeutics·Yingqun Wang
Feb 5, 2010·Cancer Metastasis Reviews·Liqin Du, Alexander Pertsemlidis
Sep 10, 2011·Therapeutic Advances in Medical Oncology·Thierry BerghmansJean-Paul Sculier
Jul 23, 2013·Cell·Radhika SubramanianTarun M Kapoor

❮ Previous
Next ❯

Citations

Mar 5, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Shuo ZhangHui Ren
Feb 18, 2016·Expert Opinion on Therapeutic Targets·Jiali YangXiaoming Liu
May 30, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Burçin Tezcanlı KaymazBuket Kosova
Sep 1, 2016·PloS One·Yushun GaoShugeng Gao
Jun 1, 2018·BioMed Research International·Yan-Zi SunShao-Ming Wang
May 8, 2018·Molecular and Clinical Oncology·Jie WangTeng Chen
May 28, 2019·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Ana Rita Thomazela MachadoLusânia Maria Greggi Antunes
Oct 21, 2020·Journal of Cellular Physiology·Heng Wee TanAndy T Y Lau
Mar 13, 2021·Journal of Ovarian Research·Parinaz ZivarpourJamal Hallajzadeh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Cancer Epigenetics & Methyl-CpG (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. Here is the latest research on cancer epigenetics and methyl-CpG binding proteins including ZBTB38.

Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Angiogenesis Inhibitors to Treat Cancer

Cancer treatments including angiogenesis inhibitors prevent tumor cells from receiving nutrients and oxygen. Here is the latest research on angiogenesis inhibitors for the treatment of cancer.

Cancer Epigenetics

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. Here is the latest research on cancer epigenetics.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.

Cell Signaling & Cancer Epigenetics (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. This feed covers the latest research on signaling and epigenetics in cell growth and cancer.

© 2021 Meta ULC. All rights reserved