MicroRNAs determine human intestinal epithelial cell fate.

Differentiation; Research in Biological Diversity
Guillaume DalmassoDidier Merlin

Abstract

MicroRNAs (miRNAs) are small, non-coding RNA molecules that post-transcriptionally regulate gene expression. Evidence has shown that miRNAs play important roles in various cellular processes, including proliferation, differentiation and survival. The intestinal epithelium is regenerated throughout life, and enterocytes undergo differentiation during migration along the crypt/villus axis. Our study aimed at establishing the expression profiles of miRNAs during intestinal epithelial cell (IEC) differentiation and determining a miRNA "signature" that distinguishes between small and large IECs. MiRNA arrays were employed to profile miRNA expression in two IEC models: the enterocyte-like Caco2-BBE and the colonocyte-like HT29-Cl.19A cell lines. Microarray data showed that in both cell lineages, the differentiated stage exhibited a different miRNA expression profile from undifferentiated stage. Interestingly, Caco2-BBE cells were distinguished from HT29-Cl.19A cells by their unique miRNA expression profile. Notably, HT29-Cl.19A cells exhibited down-regulation of miR-1269 and up-regulation of miR-99b and miR-125a-5p compared with Caco2-BBE cells. Most importantly, transfection of Caco2-BBE cells with mature miR-99b, mature miR-125a-5p...Continue Reading

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Citations

Feb 22, 2012·Development, Growth & Differentiation·Erhan AstarciSreeparna Banerjee
Apr 14, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Moiz A CharaniaDidier Merlin
Sep 8, 2012·American Journal of Respiratory and Critical Care Medicine·Owen D SolbergPrescott G Woodruff
Jan 10, 2013·BMC Gastroenterology·Cameron M SmithDamian J Hussey
May 12, 2011·PloS One·Guillaume DalmassoDidier Merlin
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Dec 1, 2013·Annals of the American Thoracic Society·Prescott G Woodruff
Aug 26, 2014·Immunology Letters·Colleen J Winstead
Oct 30, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Guillaume DalmassoDidier Merlin
May 10, 2018·Bioscience, Biotechnology, and Biochemistry·Fumina Ohsaka, Kei Sonoyama
Mar 14, 2019·Lymphatic Research and Biology·Jiwon HongAnthony R Phillips
Mar 11, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuhua ZhuJunjun Wang
May 3, 2019·American Journal of Physiology. Cell Physiology·Arivarasu N AnbazhaganPradeep K Dudeja
Nov 21, 2017·World Journal of Gastroenterology : WJG·Finbarr O'SullivanRichard Murphy
Nov 8, 2017·Inflammopharmacology·Priyanka MishraBhuvnesh Kumar
Aug 4, 2020·Frontiers in Genetics·María Belén Ruiz-RosoAlberto Dávalos
Jul 6, 2019·Frontiers in Endocrinology·Krisztina BanfaiKrisztian Kvell
Aug 19, 2021·Critical Reviews in Microbiology·Deepansh ModyVibha Rani

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