MicroRNAs from saliva of anopheline mosquitoes mimic human endogenous miRNAs and may contribute to vector-host-pathogen interactions

Scientific Reports
Bruno ArcàFabrizio Lombardo

Abstract

During blood feeding haematophagous arthropods inject into their hosts a cocktail of salivary proteins whose main role is to counteract host haemostasis, inflammation and immunity. However, animal body fluids are known to also carry miRNAs. To get insights into saliva and salivary gland miRNA repertoires of the African malaria vector Anopheles coluzzii we used small RNA-Seq and identified 214 miRNAs, including tissue-enriched, sex-biased and putative novel anopheline miRNAs. Noteworthy, miRNAs were asymmetrically distributed between saliva and salivary glands, suggesting that selected miRNAs may be preferentially directed toward mosquito saliva. The evolutionary conservation of a subset of saliva miRNAs in Anopheles and Aedes mosquitoes, and in the tick Ixodes ricinus, supports the idea of a non-random occurrence pointing to their possible physiological role in blood feeding by arthropods. Strikingly, eleven of the most abundant An. coluzzi saliva miRNAs mimicked human miRNAs. Prediction analysis and search for experimentally validated targets indicated that miRNAs from An. coluzzii saliva may act on host mRNAs involved in immune and inflammatory responses. Overall, this study raises the intriguing hypothesis that miRNAs inject...Continue Reading

References

Oct 5, 2002·Science·Robert A HoltStephen L Hoffman
Jun 27, 2003·Insect Biochemistry and Molecular Biology·Jesus G ValenzuelaJosé M C Ribeiro
May 19, 2004·Insect Biochemistry and Molecular Biology·José M C RibeiroJesus G Valenzuela
Oct 27, 2004·PLoS Biology·Bino JohnDebora S Marks
Apr 27, 2005·Nature Reviews. Molecular Cell Biology·V Narry Kim
Jun 2, 2005·Nature Reviews. Immunology·Fernando Macian
Aug 30, 2005·Proteomics·Dário E KalumeAkhilesh Pandey
Oct 11, 2005·The Journal of Experimental Biology·Bruno ArcàJosé M C Ribeiro
Jan 25, 2007·Insect Biochemistry and Molecular Biology·Bruno ArcàJosé M C Ribeiro
Jan 25, 2007·Insect Biochemistry and Molecular Biology·Eric CalvoJosé M C Ribeiro
Mar 7, 2007·Infection and Immunity·Michael J DonovanMary Ann McDowell
Sep 26, 2007·Nature Genetics·Michael KerteszEran Segal
Oct 16, 2007·Nucleic Acids Research·Flore WinterChristine Brunel
Mar 18, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·Bradley S Schneider, Stephen Higgs
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Ivo M B FrancischettiJose M C Ribeiro
May 16, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Satish L DeshmaneBassel E Sawaya
Nov 3, 2009·Proteomics·Ladawan WasinpiyamongkolDorothée Missé
Nov 21, 2009·Toxicon : Official Journal of the International Society on Toxinology·John F Andersen
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
Mar 18, 2010·BMC Bioinformatics·José Afonso Guerra-Assunção, Anton J Enright
Apr 21, 2010·Journal of the American Mosquito Control Association·Sheri L AndersonChelsea T Smartt
Jun 1, 2010·BMC Bioinformatics·Martin SturmDmitrij Frishman
Jun 11, 2010·Annual Review of Biochemistry·Marc Robert FabianWitold Filipowicz
Sep 18, 2010·Clinical Chemistry·Jessica A WeberKai Wang
Apr 21, 2011·Nature Communications·María MittelbrunnFrancisco Sánchez-Madrid
Jul 7, 2011·Current Protocols in Molecular Biology·Martha F Kramer
Jul 26, 2011·Database : the Journal of Biological Databases and Curation·Rhoda J KinsellaPaul Flicek
Sep 29, 2011·Parasites & Vectors·Albin FontaineLionel Almeras
Nov 26, 2011·Algorithms for Molecular Biology : AMB·Ronny LorenzIvo L Hofacker
Nov 30, 2011·European Journal of Immunology·Wolfgang W LeitnerTonu Wali
Dec 3, 2011·Nucleic Acids Research·Karine MegyUNKNOWN VectorBase Consortium
Dec 6, 2011·Annual Review of Immunology·Jonathan D PowellMaureen R Horton
Apr 24, 2012·Innate Immunity·Erik B WendlandtMary E Wilson
Dec 12, 2012·Nucleic Acids Research·Jiyuan AnColleen C Nelson
Jan 12, 2013·PLoS Pathogens·Rodney P Kincaid, Christopher S Sullivan

❮ Previous
Next ❯

Citations

Aug 1, 2019·Experimental and Therapeutic Medicine·Wenwen ZhouYanhong Yang
Dec 11, 2020·Developmental and Comparative Immunology·Chinmay V Tikhe, George Dimopoulos
Jul 9, 2021·Frontiers in Cellular and Infection Microbiology·Tie-Long XuBin Zheng
Jul 11, 2021·Communications Biology·Hugo D PerdomoSassan Asgari

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-Seq
dissection
Reverse-Transcription
PCR
RNAseq

Software Mentioned

miRDeep
cutadapt
BEDTools
PITA
BioMart
FastQC
BLAST
BLASTn
Bowtie
RNAfold

Related Concepts