MicroRNAs from urinary extracellular vesicles are non-invasive early biomarkers of diabetic nephropathy in type 2 diabetes patients with the 'Asian Indian phenotype'

Diabetes & Metabolism
Paramasivam PrabuMuthuswamy Balasubramanyam

Abstract

MicroRNAs (miRNAs) from extracellular vesicles (EVs) have been proposed as promising biomarkers for a number of diseases. In this study, their potential as urine-based biomarkers of diabetic nephropathy (DN) was assessed. MiRNAs were profiled in urinary EVs from 160 fasting subjects with normal glucose tolerance (NGT) and in T2DM patients with either microalbumininuria (MIC) or macroalbuminuria (MAC). A total of 73 miRNAs detected in urinary EVs (NGT) were predicted to target important functions for kidney homoeostasis, thereby validating their use as indicators of kidney dysfunction. Indeed, a urinary EV miRNA signature was found to comprise increased levels of let-7i-3p, miR-24-3p and miR-27b-3p, and decreased levels of miR-15b-5p, to identify patients with MIC. ROC curve analysis confirmed this ability to identify MIC in normo-albuminuria T2DM (T2DM-NA) patients and to differentiate between MAC and T2DM patients. These miRNAs were also predicted to target protein networks involved in the Wnt/β-catenin signalling cascade, activin receptor signalling and cell differentiation/proliferation, and correlated with eGRF, HbA1c, serum creatinine, urea, albumin and blood pressure. Concentrations of miR-30a-5p were specifically modifie...Continue Reading

Citations

Apr 30, 2020·American Journal of Physiology. Cell Physiology·Nicole Noren Hooten, Michele K Evans
Jul 14, 2020·Journal of Diabetes Research·Jiarong LvShizhong Bu
Jun 26, 2020·Frontiers in Immunology·Yanfang LuZhangsuo Liu
Jul 25, 2019·Journal of Translational Medicine·Hyoungnae KimSoon Hyo Kwon
Aug 9, 2020·Clinical and Experimental Nephrology·Wei ZhangHao Zhang
May 3, 2019·The Korean Journal of Internal Medicine·Soon Hyo Kwon
Feb 3, 2021·Acta Diabetologica·Federica BaruttaGabriella Gruden
Jan 13, 2021·International Journal of Molecular Sciences·Yong-Hui JiangShi-Gang Zhao
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Jun 3, 2021·Journal of Clinical Medicine·Goren Saenz-PipaonCarmen Roncal
Apr 20, 2021·Kidney Research and Clinical Practice·Sul A LeeTae-Hyun Yoo
Mar 17, 2020·Computers in Biology and Medicine·Turki Turki, Y-H Taguchi
Sep 3, 2021·Stem Cells International·Can JinHui Qian

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