MicroRNAs in Small Extracellular Vesicles Indicate Successful Embryo Implantation during Early Pregnancy.

Cells
Qiang TanZhengguang Wang

Abstract

Synchronous communication between the developing embryo and the receptive endometrium is crucial for embryo implantation. Thus, uterine receptivity evaluation is vital in managing recurrent implantation failure (RIF). The potential roles of small extracellular vesicle (sEV) miRNAs in pregnancy have been widely studied. However, the systematic study of sEVs derived from endometrium and its cargos during the implantation stage have not yet been reported. In this study, we isolated endometrium-derived sEVs from the mouse endometrium on D2 (pre-receptive phase), D4 (receptive phase), and D5 (implantation) of pregnancy. Herein, we reveal that multivesicular bodies (MVBs) in the endometrium increase in number during the window of implantation (WOI). Moreover, our findings indicate that CD63, a well-known sEV marker, is expressed in the luminal and glandular epithelium of mouse endometrium. The sEV miRNA expression profiles indicated that miR-34c-5p, miR-210, miR-369-5p, miR-30b, and miR-582-5p are enriched during WOI. Further, we integrated the RIF's database analysis results and found out that miR-34c-5p regulates growth arrest specific 1 (GAS1) for normal embryo implantation. Notably, miR-34c-5p is downregulated during implantation...Continue Reading

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Citations

Jan 21, 2021·Journal of Assisted Reproduction and Genetics·Ronit MachtingerHaotian Wu
Nov 17, 2020·Frontiers in Cell and Developmental Biology·Florian LangMadhuri S Salker
Jan 21, 2021·Frontiers in Veterinary Science·Bahram TalebjediMarcia De Almeida Monteiro Melo Ferraz
May 30, 2021·Molecular Human Reproduction·Upendra Kumar SoniRajesh Kumar Jha

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Datasets Mentioned

BETA
GSE92324
GSE111974
GSE71332

Methods Mentioned

BETA
biopsy
light microscopy
confocal microscopy
Protein Assay
chips
chip

Software Mentioned

ZetaView
R
NIS Elements
GraphPad Prism
Limma
R basic
ZEN
NTA

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