MicroRNAs miR-25, let-7 and miR-124 regulate the neurogenic potential of Müller glia in mice

Development
Stefanie G WohlThomas A Reh

Abstract

Müller glial cells (MG) generate retinal progenitor (RPC)-like cells after injury in non-mammalian species, although this does not occur in the mammalian retina. Studies have profiled gene expression in these cells to define genes that may be relevant to their differences in neurogenic potential. However, less is known about differences in micro-RNA (miRNA) expression. In this study, we compared miRNAs from RPCs and MG to identify miRNAs more highly expressed in RPCs, and others more highly expressed in MG. To determine whether these miRNAs are relevant to the difference in neurogenic potential between these two cell types, we tested them in dissociated cultures of MG using either mimics or antagomiRs to increase or reduce expression, respectively. Among the miRNAs tested, miR-25 and miR-124 overexpression, or let-7 antagonism, induced Ascl1 expression and conversion of ∼40% of mature MG into a neuronal/RPC phenotype. Our results suggest that the differences in miRNA expression between MG and RPCs contribute to their difference in neurogenic potential, and that manipulations in miRNAs provide a new tool with which to reprogram MG for retinal regeneration.

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Citations

Oct 28, 2019·Scientific Reports·Corinne L A FairchildAnna La Torre
Sep 27, 2019·Current Eye Research·Rahul Langhe, Rachael A Pearson
Jan 9, 2021·Molecular Neurobiology·Hui GaoHaiwei Xu
Dec 17, 2020·Cell Reports·Levi ToddThomas A Reh
Jan 8, 2021·Experimental Eye Research·Priscilla Sayami AkamineDânia Emi Hamassaki
Dec 18, 2019·Progress in Retinal and Eye Research·Iqbal AhmadXiaohuan Xia
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Julia Sophie PawlickVolker Busskamp
Feb 5, 2021·Frontiers in Cell and Developmental Biology·Gregory J KonarJames G Patton
Jul 4, 2020·Current Opinion in Genetics & Development·Diana García-GarcíaMuriel Perron
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Elizabeth S FishmanAnna La Torre

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