MicroRNAs Regulated by the LPS/TLR2 Immune Axis as Bona Fide Biomarkers for Diagnosis of Acute Leptospirosis.

MSphere
Charles Solomon Akino MercyKalimuthusamy Natarajaseenivasan

Abstract

Leptospirosis remains a significant human health issue due to its systemic complications. Therefore, biomarkers that are more effective are urgently needed for the early diagnosis of leptospirosis. MicroRNAs (miRNAs) are evolutionarily conserved regulatory RNAs that have shown the potential to be used as biomarkers for diagnosis, prognosis, and therapy of infectious diseases. In this study, we performed an unbiased screen using the miRNome miRNA array to identify circulating miRNAs with the potential to serve as authentic biomarkers for early diagnosis of leptospirosis. Because leptospiral lipopolysaccharide (LPS) is the predominant leptospiral antigen and plays a vital role in immunological and biological activities, we used LPS treated and untreated in vitro (THP1 cells) and in vivo (BALB/c mice) surrogate models to identify the LPS-specific miRNAs. Differential expression analysis revealed 18 miRNAs to be associated strongly with LPS stimulation in THP1 cells. Of these, three (miR-let-7b-5p, miR-144-3p, and miR-21-5p) were observed to be present at increased levels in vivo The identified miRNAs were validated for their biomarker potential using serum samples from leptospirosis-negative patients and patients with confirmed ca...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1978·Journal of Medical Microbiology·B Adler, S Faine
Apr 1, 1992·Canadian Journal of Microbiology·L de Souza, M C Koury
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jan 1, 1995·Annual Review of Immunology·R J Ulevitch, P S Tobias
Feb 4, 1999·Nature Medicine·R J Ulevitch
Mar 6, 1999·Current Opinion in Microbiology·R P Darveau
Nov 5, 1999·Nature·R J Ulevitch
Feb 19, 2000·Current Opinion in Immunology·B Beutler
Dec 4, 2003·The Lancet Infectious Diseases·Ajay R BhartiUNKNOWN Peru-United States Leptospirosis Consortium
Jul 28, 1951·Nature·M DUBOISF SMITH
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marie-Anne NahoriCatherine Werts
Jun 6, 2006·PLoS Genetics·Xionglei He, Jianzhi Zhang
Aug 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Konstantin D TaganovDavid Baltimore
Oct 3, 2006·Developmental Cell·Wigard P Kloosterman, Ronald H A Plasterk
Jul 23, 2008·Nature Immunology·David BaltimoreKonstantin D Taganov
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Sep 6, 2008·PloS One·Shlomit GiladAyelet Chajut
Dec 17, 2008·Annals of the Rheumatic Diseases·F J Sheedy, L A J O'Neill
Jan 10, 2009·Annual Review of Immunology·Cedric AuffrayFrederic Geissmann
Jan 13, 2009·Cell·Changchun Xiao, Klaus Rajewsky
Feb 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maurizio CeppiPhilippe Pierre
Mar 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Flavia BazzoniMassimo Locati
May 12, 2009·Expert Opinion on Biological Therapy·Maria Angelica Cortez, George Adrian Calin
Dec 1, 2009·Nature Reviews. Microbiology·Clare E BryantNicholas J Gay
Jul 10, 2010·Nucleic Acids Research·Kai WangDavid J Galas
Nov 9, 2010·Communicative & Integrative Biology·Haruhisa IguchiTakahiro Ochiya
Feb 19, 2011·Nature Reviews. Immunology·Luke A O'NeillClaire E McCoy
Mar 16, 2011·Mutation Research·Alton EtheridgeKai Wang
Jun 8, 2011·Nature Reviews. Clinical Oncology·Maria Angelica CortezGeorge A Calin
Oct 5, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marian L TurnerThomas Brocker
Oct 11, 2011·Nature Reviews. Immunology·Chao Shi, Eric G Pamer
Oct 27, 2011·Cardiovascular Research·Anna ZampetakiManuel Mayr
Jan 21, 2012·Trends in Cell Biology·Xi ChenChen-Yu Zhang
Aug 2, 2012·Journal of Biomedical Science·Ching-Hua HsiehJohnson Chia-Shen Yang
Dec 4, 2012·Nucleic Acids Research·Andrea FranceschiniLars J Jensen
Mar 4, 2014·PloS One·Hongtai ZhangGuangming Zhou
Nov 13, 2014·Current Topics in Microbiology and Immunology·David A Haake, Paul N Levett
Feb 7, 2015·The European Respiratory Journal·Irene LatorreAndreas Meyerhans
Nov 27, 2015·Clinical and Vaccine Immunology : CVI·Veerapandian RajaKalimuthusamy Natarajaseenivasan

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Methods Mentioned

BETA
PCR
ELISA
electrophoresis
transfection
protein assay

Software Mentioned

Metascape
SigmaPlot
Cytoscape
miRNome
TargetScan
microT
CDS
GraphPad Prism
DIANA

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