Microsatellite-centromere mapping in the zebrafish (Danio rerio)

Genomics
E J KauffmanK C Cheng

Abstract

Ten (CA)n microsatellite simple sequence repeat (SSR) markers, 1, 2, 12, 14, 16, 18, 20, 22, 26, and 29, were used to show high chiasma interference and to determine centromere-marker map distances in the zebrafish (Danio rerio). Of these, SSR 12 exhibited no recombinant tetratypes among 175 half-tetrad embryos, placing this marker within 1 cM of the centromere of Linkage Group XVII. Fractions of heterozygous half-tetrads for the remaining nine markers ranged from 0.64 to 0.89. Of these, six recombinant fractions were more than 0.67 (P < 0.05), indicating strong chiasma interference during female meiosis in the zebrafish. Consistent with previous mapping data, SSRs 2 and 20 of Linkage Group VI were tightly linked. Half-tetrad analysis will allow the mapping of the remaining centromeres and may be useful in the mapping of new genes and mutations in the zebrafish.

Citations

Jul 11, 2006·Cytogenetic and Genome Research·R B PhillipsJ H Postlethwait
Aug 28, 2007·Genetics·Darrin P ReidMichael Reith
Oct 29, 2010·Genetics·Bradley L DemarestNikolaus S Trede
Oct 28, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Manzoor-Ali P K MohideenKeith C Cheng
Aug 12, 2014·Marine Biotechnology·Ma Jesús Molina-LuzónRoberto de la Herrán
May 15, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mustafa K KhokhaLyle B Zimmerman

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