Microsatellite Instability and Altered Expressions of MLH1 and MSH2 in Gastric Cancer

Asian Pacific Journal of Cancer Prevention : APJCP
Nor Hasyimah HaronIsa Mohamed Rose

Abstract

Introduction: Microsatellite instability (MSI) is a hallmark of defective DNA mismatch repair (MMR) of genes especially MLH1 and MSH2. It is frequently involved in the carcinogenesis of various tumours including gastric cancer (GC). However, MSI in GCs have not been reported in Malaysia before. Objective: This study was conducted to determine the microsatellite instability (MSI) status in gastric cancer by microsatellite analysis, sequencing, its association with MLH1 and MSH2 protein expression and H.pylori infection by immunohistochemistry. Method: A total of 60 gastric cancer cases were retrieved. DNA was extracted from paired normal and tumour tissues while MLH1 and MSH2 protein expression as well as H. pylori status were determined by IHC staining. For microsatellite analysis, polymerase chain reaction (PCR) was performed for paired tissue samples using a panel of five microsatellite markers. MSI-positive results were subjected for DNA sequencing to assess mutations in the MLH1 and MSH2 genes. Results: Microsatellite analysis identified ten MSI positive cases (16.7%), out of which only six cases (10.3%) showed absence of MLH1 (n=3) or MSH2 (n=3) protein expression by IHC. The most frequent microsatellite marker in MSI posi...Continue Reading

Citations

Jul 30, 2020·Mediators of Inflammation·Salah A Sheweita, Awad S Alsamghan
Apr 8, 2020·Computational and Structural Biotechnology Journal·Lin LiXiaosheng Wang
Oct 30, 2020·Cancer Management and Research·Meiying CuiWencai Li
Feb 12, 2021·Cancers·Matthew Man-Kin WongSung Kay Chiu

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Methods Mentioned

BETA
PCR

Software Mentioned

Genotyper Analysis Software
Pmut
Gene Mapper

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