Abstract
Microsatellite instability (MI) and loss of heterozygosity (LOH) are described in lung cancer specimens. However, their importance in tumorigenesis remains unknown. The aim of this study was to identify the presence of MI and LOH in human tumor and normal bronchial mucosa DNA. We performed biopsies with fiberoptic bronchoscopy and took specimens from the tumor and from the opposite site normal bronchial mucosa in 20 patients with non-small-cell lung cancer (NSCLC). Four patients had an adenocarcinoma, while 16 had a squamous cell carcinoma. Also, 6 patients had an early-stage disease (stages I and II), while 14 patients had an advanced-stage disease (stages III and IV). All paired specimens were studied for MI and LOH on chromosome 17p, 17q, 9p, and 9q, with 10 polymorphic markers. Sixteen of 20 tumors displayed genetic alterations (80%). Six tumors (30%) exhibited MI, five tumors (25%) exhibited LOH, while five tumors exhibited MI and LOH concurrently. The marker HBX had the most frequent incidence of LOH (4/20, 20%), indicating that the hbx gene becomes a strong candidate tumor suppressor gene, whereas of MI it was D17S515 (4/20, 20%). No relationship was observed between the presence of LOH or MI and the histologic subtype o...Continue Reading
References
Jan 1, 1991·Genes, Chromosomes & Cancer·B JohanssonF Mitelman
Apr 1, 1986·Chest·C F Mountain
Aug 16, 1995·Journal of the National Cancer Institute·Y KishimotoA F Gazdar
Oct 20, 1995·Cancer Letters·H KiarisD A Spandidos
Jan 1, 1995·Cancer Research·K M FongP J Smith
Apr 1, 1993·Chest·J D Minna
May 7, 1993·Science·L A AaltonenS R Hamilton
May 15, 1996·Cancer Research·M MiozzoM A Pierotti
Citations
Nov 21, 2001·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·J ZhouM S Tockman
Mar 28, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Demetrios A ArvanitisDemetrios A Spandidos
Apr 2, 2003·Cancer Letters·Fung Mei KwongMaria Li Lung
Feb 4, 1999·Oral Oncology·E RizosD A Spandidos
Oct 12, 2010·Carcinogenesis·Yu-Jing HuangQingyi Wei
Aug 18, 2012·Pulmonary Medicine·Kostas ArchontogeorgisDemosthenes Bouros
Nov 11, 1999·American Journal of Respiratory and Critical Care Medicine·D A VassilakisD Bouros
Sep 16, 2000·American Journal of Respiratory and Critical Care Medicine·D A VassilakisD Bouros
Jun 11, 2005·American Journal of Respiratory and Critical Care Medicine·Giovanna E CarpagnanoAngelo Paradiso
Nov 4, 2000·American Journal of Respiratory Cell and Molecular Biology·Y G ManJ L Mulshine
May 16, 2012·Respiration; International Review of Thoracic Diseases·Marios E Froudarakis
Aug 10, 2002·Journal of Cellular and Molecular Medicine·K DemopoulosD A Spandidos
Aug 21, 2003·Environmental and Molecular Mutagenesis·Nazumi A YamadaRosann A Farber
Apr 2, 2008·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Vienna LudoviniMaurizio Tonato
Jul 29, 1999·Chest·N M SiafakasD Spandidos
Dec 8, 2015·Virchows Archiv : an International Journal of Pathology·Arne WarthMatthias Kloor
Sep 4, 2009·Pediatric Pulmonology·Christina ThomouEleni G Tzortzaki
Sep 6, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Eleni G TzortzakiNikolaos M Siafakas
Jul 12, 2005·Respiratory Medicine·Katerina SamaraEleni G Tzortzaki
Oct 1, 2016·Medical Oncology·G E CarpagnanoM P Foschino Barbaro
Mar 1, 2002·Molecular Carcinogenesis·Toshiyuki HiroseYasumasa Monden
Jun 3, 2004·International Journal of Surgical Pathology·Alberto CavazzaGiulio Rossi
Mar 5, 2003·Pharmacological Reviews·Romano DanesiMario Del Tacca
Jan 23, 2003·Journal of the American Society of Nephrology : JASN·Jean GogusevDominique Droz