Microscale Electro-Hydrodynamic Cell Printing with High Viability

Small
Jiankang HeDichen Li

Abstract

Cell printing has gained extensive attentions for the controlled fabrication of living cellular constructs in vitro. Various cell printing techniques are now being explored and developed for improved cell viability and printing resolution. Here an electro-hydrodynamic cell printing strategy is developed with microscale resolution (<100 µm) and high cellular viability (>95%). Unlike the existing electro-hydrodynamic cell jetting or printing explorations, insulating substrate is used to replace conventional semiconductive substrate as the collecting surface which significantly reduces the electrical current in the electro-hydrodynamic printing process from milliamperes (>0.5 mA) to microamperes (<10 µA). Additionally, the nozzle-to-collector distance is fixed as small as 100 µm for better control over filament deposition. These features ensure high cellular viability and normal postproliferative capability of the electro-hydrodynamically printed cells. The smallest width of the electro-hydrodynamically printed hydrogel filament is 82.4 ± 14.3 µm by optimizing process parameters. Multiple hydrogels or multilayer cell-laden constructs can be flexibly printed under cell-friendly conditions. The printed cells in multilayer hydrogels ...Continue Reading

References

Apr 13, 2006·Nano Letters·Daoheng SunLiwei Lin
Sep 8, 2011·Proceedings of the National Academy of Sciences of the United States of America·Simonetta GrilliPietro Ferraro
Nov 19, 2011·Advanced Materials·Toby D BrownDietmar W Hutmacher
Nov 20, 2012·Science·Brian Derby
Apr 12, 2013·Chemical Society Reviews·Savas TasogluUtkan Demirci
Jul 31, 2013·Small·Samantha L SampsonBrian D Robertson
Apr 2, 2014·Advanced Materials·Minxuan KuangYanlin Song
Jun 7, 2014·Tissue Engineering. Part C, Methods·Luca GasperiniClaudio Migliaresi
Jul 25, 2014·The Analyst·Elisabeth Ehler, Suwan N Jayasinghe
Aug 6, 2014·Nature Biotechnology·Sean V Murphy, Anthony Atala
Apr 29, 2015·Nature Communications·Jetze VisserJos Malda
Apr 16, 2016·Annals of Biomedical Engineering·Jeong Hun ParkDong-Woo Cho
Jun 1, 2016·Soft Matter·L MecozziP Ferraro
Aug 2, 2016·Nanoscale·Bing ZhangDichen Li
Oct 21, 2016·Macromolecular Bioscience·Laura MecozziSimonetta Grilli
Jan 21, 2017·Tissue Engineering. Part B, Reviews·Tanveer Ahmad Mir, Makoto Nakamura
Mar 23, 2017·Journal of Medical Ethics·Niki VermeulenWenmiao Shu
Jan 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kaimin ChenHong Xu

❮ Previous
Next ❯

Citations

Oct 1, 2020·Journal of Biomaterials Applications·Wenjie YeAiguo Ouyang
Nov 30, 2019·The Review of Scientific Instruments·Kai LiMarc Madou
Jun 17, 2020·Advanced Science·Paul D DaltonJürgen Groll
Oct 6, 2020·Macromolecular Bioscience·Jaideep AdhikariProsenjit Saha
Mar 5, 2021·Biofabrication·Guy DecanteJ Miguel Oliveira
Jun 8, 2021·Frontiers in Surgery·Xingdou MuYue Jiang
May 14, 2019·ACS Applied Materials & Interfaces·Elisabeth L GillYan Yan Shery Huang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.