Microscale magnetic field modulation for enhanced capture and distribution of rare circulating tumor cells

Scientific Reports
Peng ChenJohn X J Zhang

Abstract

Immunomagnetic assay combines the powers of the magnetic separation and biomarker recognition and has been an effective tool to perform rare Circulating Tumor Cells detection. Key factors associated with immunomagnetic assay include the capture rate, which indicates the sensitivity of the system, and distributions of target cells after capture, which impact the cell integrity and other biological properties that are critical to downstream analyses. Here we present a theoretical framework and technical approach to implement a microscale magnetic immunoassay through modulating local magnetic field towards enhanced capture and distribution of rare cancer cells. Through the design of a two-dimensional micromagnet array, we characterize the magnetic field generation and quantify the impact of the micromagnets on rare cell separation. Good agreement is achieved between the theory and experiments using a human colon cancer cell line (COLO205) as the capture targets.

References

Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D S HsiehJ Folkman
Oct 15, 1995·Analytical Chemistry·M ZborowskiJ J Chalmers
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C FanelliL Ghibelli
Jan 26, 2002·Science·Abraham D StroockGeorge M Whitesides
Mar 19, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Tsunehisa KimuraAkihiro Nara
Nov 18, 2006·Cell·Gaorav P Gupta, Joan Massagué
Apr 27, 2010·Analytical and Bioanalytical Chemistry·Daniel R GossettDino Di Carlo
Jun 30, 2010·Analytical Chemistry·Masahito HosokawaTadashi Matsunaga
Oct 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shannon L StottMehmet Toner
Oct 28, 2010·Biomedical Microdevices·Siyang ZhengYu-Chong Tai
Jun 22, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Sangjo ShimKatherine Stemke Hale
Aug 25, 2011·Lab on a Chip·Kazunori HoshinoXiaojing Zhang
Oct 13, 2011·Journal of the American Chemical Society·Christine E ProbstXiaohu Gao
Nov 7, 2012·Cancer Metastasis Reviews·François-Clément BidardJean-Yves Pierga
Mar 13, 2013·Applied Physics Letters·Dharmakeerthi NawarathnaMichelle Khine
Sep 11, 2013·ACS Nano·Chun-Hsien WuKonstantin V Sokolov
Jan 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael J MitchellMichael R King
Oct 1, 2012·Microfluidics and Nanofluidics·Taotao ZhuLeidong Mao

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Citations

Nov 17, 2015·Analytical Chemistry·Sanjin HosicAbigail N Koppes
Dec 9, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Amogha TadimetyJohn X J Zhang
Dec 22, 2016·Lab on a Chip·Ran ZhouCheng Wang
Jul 12, 2017·ACS Applied Materials & Interfaces·Mahla PoudinehShana O Kelley
Feb 2, 2018·Critical Reviews in Clinical Laboratory Sciences·Amogha TadimetyJohn X J Zhang
Jun 12, 2018·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Fadi AlnaimatSaud Khashan
Apr 27, 2018·Chemistry : a European Journal·Mashooq KhanJin-Ming Lin
Feb 20, 2019·Electrophoresis·Yuanyuan MaLiqiang Luo
Aug 21, 2015·Annals of Biomedical Engineering·Peng ChenXiaojing Zhang
Apr 24, 2020·Cancer Medicine·Laan Luo, Yongqing He
Feb 1, 2018·Proceedings of the Institution of Mechanical Engineers. Part C. Journal of Mechanical Engineering Science·Jifu TanYaling Liu
May 26, 2018·The Analyst·Hyungseok ChoKi-Ho Han
Dec 13, 2017·BioMed Research International·Yoon Seok SuhKang Hyun Lee
Jan 9, 2019·Frontiers in Bioengineering and Biotechnology·Sena YamanH Cumhur Tekin
Dec 2, 2020·Cancers·Peng LiuMichiel Stevens
Jul 3, 2021·Diagnostics·Michiel StevensLeon Terstappen
Oct 23, 2021·ACS Biomaterials Science & Engineering·Kazuhiko IshiharaKyoko Fukazawa

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