Abstract
Currently, albendazole is one of the most commonly used drugs because of its affordability. The objective was to evaluate the histopathology of Fasciola hepatica specimens. For this, the efficacy test was performed on sheep treated with albendazole at the dose recommended for F. hepatica, in which the helminths recovered at necropsy were counted and separated for histology. Spermatogenic cells from parasites recovered from treated and control sheep were examined by microscopy. The fecal egg-count reduction test revealed 97.06% efficacy of albendazole in the treatment of F. hepatica. Changes in testicular tubule cells started 48 hours after treatment and became evident within 72 hours, at which point it became difficult to identify cell types. Primary and secondary spermatogonia became increasingly rare and intercellular vacuolization was more evident. Signs of apoptosis, with pycnotic nuclei and evidence of keriorrexia were observed at all times. Cell debris was identified 96 hours after treatment. The results indicated that parasitic spermatogenesis was severely affected by albendazole and demonstrated the importance of the use of histopathology for the diagnosis of therapeutic efficacy in field strains.
References
Sep 1, 1992·Veterinary Parasitology·G C ColesP J Waller
Dec 1, 1990·Parasitology·A W Stitt, I Fairweather
Nov 1, 1988·International Journal for Parasitology·E Lacey
May 1, 1968·Oral Surgery, Oral Medicine, and Oral Pathology
Jun 1, 1995·Veterinary Parasitology·I B WoodS M Taylor
Dec 29, 1999·Molecular and Biochemical Parasitology·D P JasmerS Johnson
Mar 15, 2003·Parasitology·J F BuchananE M Hoey
Aug 12, 2006·Veterinary Parasitology·R E B HannaA Couper
Sep 9, 2008·Veterinary Parasitology·R E B HannaR Daniel
Jun 30, 2009·Veterinary Parasitology·L AlvarezC Lanusse
Nov 19, 2009·Parasitology Research·Maeve McConvilleIan Fairweather
Jan 8, 2010·Veterinary Parasitology·R E B HannaI Fairweather
Dec 21, 2010·Veterinary Parasitology·S ScarcellaH Solana
May 5, 2011·Parasitology Research·Emma TonerIan Fairweather
Jun 28, 2011·Veterinary Parasitology·Ian Fairweather
Sep 29, 2011·Veterinary Parasitology·R E B HannaI Fairweather
Oct 4, 2012·Geospatial Health·Isabella Vilhena Freire MartinsAdevair Henrique da Fonseca
Nov 12, 2013·Experimental Parasitology·M Martínez-ValladaresF A Rojo-Vázquez
Jul 2, 2015·Pathogens·Robert Hanna