Microscopic analysis of NADH fluorescence during aerobic and anaerobic liver preservation conditions: A noninvasive technique for assessment of hepatic metabolism

Cryobiology
H KlaukeM D Menger

Abstract

Gaseous insufflation of oxygen via the venous vascular system is thought to be an useful tool for preventing anoxic tissue injury during extended time periods of ischemic preservation and for allowing for an improved recovery of organ function after transplantation. The present study aimed at the application of a noninvasive technique for monitoring effectiveness and homogeneity of gaseous areation by using an epiillumination microscopic technique for assessment of tissue nicotinamide adenine dinucleotide (NADH) fluorescence. Rat livers were flushed with and stored in University of Wisconsin solution at 4 degrees C for 48 h (n = 20). In half of the experiments (n = 10) gaseous oxygen was applied subsequent to organ harvest. Using ultraviolet-excitation high-resolution microscopy and computer-assisted image analysis liver surfaces were scanned for NADH intensity and spatial heterogeneity at 1, 24, and 48 h preservation time. Livers simply stored without aeration served as controls (n = 10). NADH intensity data were compared with corresponding data of tissue adenosine triphosphate (ATP) concentrations determined enzymatically. NADH fluorescence already differed at 1 h preservation between the two groups with significantly lower v...Continue Reading

References

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Citations

Mar 10, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Giovanni Bottiroli, Anna Cleta Croce
Mar 13, 2014·Lasers in Surgery and Medicine·Anna C CroceMariapia Vairetti
Jun 29, 2010·Lasers in Surgery and Medicine·Anna C CroceGiovanni Bottiroli
Apr 29, 2014·Journal of Hepatology·Jay A Graham, James V Guarrera
Oct 26, 2016·Journal of Photochemistry and Photobiology. B, Biology·Anna C CroceMariapia Vairetti
Jul 28, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Anna Cleta CroceMariapia Vairetti
Jul 30, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anna Cleta CroceGiovanni Bottiroli
Aug 30, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anna Cleta CroceGiovanni Bottiroli
Nov 2, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anna Cleta CroceGiovanni Bottiroli
Apr 7, 2018·Liver International : Official Journal of the International Association for the Study of the Liver·Anna Cleta CroceMariapia Vairetti
Jul 1, 2016·World Journal of Transplantation·Paria MahboubPaulo N Martins

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