Abstract
Only eight families of double-stranded DNA (dsDNA) viruses are known to infect vertebrate animals. During an investigation of papillomatous skin disease in an elasmobranch species, the giant guitarfish (Rhynchobatus djiddensis), a novel virus, distinct from all known viral families in regard to particle size, morphology, genome organization, and helicase phylogeny was discovered. Large inclusion bodies containing 75-nm icosahedral viral particles were present within epithelial cell nuclei in the proliferative skin lesions. Deep metagenomic sequencing revealed a 22-kb circular dsDNA viral genome, tentatively named guitarfish "adomavirus" (GAdoV), with only distant homology to two other fish viruses, Japanese eel endothelial cell-infecting virus (JEECV) and a recently reported marbled eel virus. Phylogenetic analysis of the helicase domain places the guitarfish virus in a novel clade that is equidistant between members of the Papillomaviridae and Polyomaviridae families. Specific PCR, quantitative PCR, and in situ hybridization were used to detect, quantify, and confirm that GAdoV DNA was localized to affected epithelial cell nuclei. Changes in the viral titer, as well as the presence of a hybridization signal, coincided with the...Continue Reading
References
Nov 26, 1997·Journal of Virology·Q ShengB Schaffhausen
Nov 22, 2001·The American Journal of Tropical Medicine and Hygiene·J E DawsonS R Zaki
Oct 12, 2010·PLoS Biology·Clément Gilbert, Cédric Feschotte
Feb 1, 2011·Virology·Tetsuya MizutaniShin-ichi Ono
Aug 31, 2012·Journal of Virology·Terry Fei Fan NgEric Delwart
Jan 2, 2013·Ticks and Tick-borne Diseases·Cynthia M TateMichael J Yabsley
Mar 27, 2013·Veterinary Pathology·M M Garner
May 9, 2013·PLoS Currents·Ricardo Betancur-RGuillermo Ortí
Jul 16, 2013·Current Opinion in Virology·Eugene V Koonin, Valerian V Dolja
Nov 28, 2013·Emerging Infectious Diseases·Terry Fei Fan NgEric Delwart
Jul 19, 2014·Virology·Mohammad MoniruzzamanSteven W Wilhelm
Aug 15, 2014·Journal of Fish Diseases·S OkazakiT Mizutani
Oct 29, 2014·Proceedings of the National Academy of Sciences of the United States of America·Terry Fei Fan NgEric Delwart
Jan 31, 2015·Genome Announcements·Alberto PerettiChristopher B Buck
Mar 3, 2015·Annals of the New York Academy of Sciences·Eugene V KooninNatalya Yutin
Mar 6, 2015·Journal of Virology·Terry Fei Fan NgEric Delwart
Mar 12, 2015·Annals of the New York Academy of Sciences·Arshan NasirGustavo Caetano-Anollés
Nov 15, 2015·Journal of Fish Diseases·C M WenF H Nan
Jan 14, 2016·Diseases of Aquatic Organisms·Alvin CamusTerry Fei Fan Ng
Apr 20, 2016·PLoS Pathogens·Christopher B BuckArvind Varsani
May 21, 2016·Genome Announcements·Jennifer A DillAlvin C Camus
Jul 22, 2016·Journal of Virology·Alberto López-BuenoAlí Alejo
Feb 14, 2017·Virus Research·Siddharth R Krishnamurthy, David Wang
Sep 5, 2017·Cell Host & Microbe·Chris LauberRalf Bartenschlager
Citations
Jan 12, 2019·Journal of Fish Diseases·Hui-Yu PaoChiu Ming Wen
Jun 5, 2020·Diseases of Aquatic Organisms·Vicki S BlazerLuke R Iwanowicz
Apr 4, 2020·Microbiology Resource Announcements·Luke R IwanowiczR Scott Cornman
Mar 7, 2020·Microbiology and Molecular Biology Reviews : MMBR·Eugene V KooninJens H Kuhn
May 22, 2020·Journal of Virology·Esteban SotoTerry Fei Fan Ng
Jul 30, 2021·Journal of Fish Diseases·Jin-Xian YangJun-Qing Ge