Microscopic and physiologic evidence for biofilm-associated wound colonization in vivo

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Stephen C DavisP M Mertz

Abstract

A biofilm is a collection of microbial cells that are attached to a surface and embedded in a self-produced extrapolymeric substance. The understanding of the biofilm phenotype is important in the understanding of bacteria in vitro but it has been difficult to translate biofilm science to the clinical setting. More recently, preliminary criteria for defining biofilm associated diseases have been proposed and the purpose of this study was to create a biofilm-associated wound model based on these criteria. Using a porcine model, partial thickness wounds were inoculated with a wound isolate Staphylococcus aureus strain. Wounds were then treated with either one of two topical antimicrobial agents (mupriocin cream or triple antibiotic ointment) within 15 minutes to represent planktonic bacteria or 48 hours after initial inoculation to represent biofilm-associated wound infection. Using light microscopy, scanning electron microscopy and epifluorescence microscopy, we were able to observe biofilm-like structures in wounds after 48 hours of inoculation and occlusion. The in vivo antimicrobial assay was used to demonstrate that both mupirocin cream and the triple antibiotic ointment were effective in reducing planktonic S. aureus but ha...Continue Reading

References

Dec 1, 1977·Canadian Journal of Microbiology·G G GeeseyJ W Costerton
Jun 1, 1994·Current Opinion in Biotechnology·M Fletcher
Feb 5, 2000·The Journal of Hospital Infection·D Mack
Dec 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·M E Davey, G A O'toole
Sep 13, 2001·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·S C DavisP M Mertz
Jan 25, 2002·Journal of Bacteriology·Karin SauerDavid G Davies
Apr 16, 2002·Molecular Microbiology·Friedrich Götz
Sep 26, 2003·Journal of Dermatological Science·Hisanori AkiyamaKeiji Iwatsuki
Oct 7, 2003·Annual Review of Microbiology·Matthew R Parsek, Pradeep K Singh

❮ Previous
Next ❯

Citations

Apr 12, 2013·Biofouling·M C Ammons, V Copié
Mar 19, 2009·Tissue Engineering. Part C, Methods·Joanna ShepherdSheila MacNeil
Jun 1, 2012·Advances in Wound Care·Christopher Attinger, Randy Wolcott
Nov 18, 2008·The Journal of Antimicrobial Chemotherapy·Kristina FlemmingTrond Flaegstad
Apr 10, 2010·The Journal of Antimicrobial Chemotherapy·Katja E HillDavid W Thomas
Nov 26, 2009·Plastic and Reconstructive Surgery·Rod J RohrichSteven Fagien
Jan 30, 2013·Plastic and Reconstructive Surgery·Akhil K SethSeok J Hong
Jan 29, 2013·Journal of Wound, Ostomy, and Continence Nursing : Official Publication of the Wound, Ostomy and Continence Nurses Society·Beth Hawkins Bradley, Muriel Cunningham
Jun 6, 2009·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Carlos A CharlesRobert S Kirsner
Sep 22, 2010·Antimicrobial Agents and Chemotherapy·Victoria KostenkoRobert John Martinuzzi
Apr 30, 2013·Journal of Bacteriology·Susanne Haussler, Clay Fuqua
Oct 9, 2009·Journal of Clinical Microbiology·Mustafa FazliTim Tolker-Nielsen
Mar 20, 2009·Canadian Journal of Microbiology·Karen FreemanChristine A Cochrane
Feb 9, 2010·Journal of Dental Research·S Guo, L A Dipietro
Jan 30, 2013·BMC Infectious Diseases·Carlos J SanchezClinton K Murray
Jan 30, 2013·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Crystal HolmesBlaise R Boles
Jul 8, 2010·Journal of Cutaneous and Aesthetic Surgery·Anitha B Sadashivaiah, Venkataram Mysore
Feb 27, 2014·Journal of the American Veterinary Medical Association·Elizabeth A SwansonPaul W Snyder
Apr 5, 2013·BioMed Research International·Agústa GudmundsdóttirBjarki Stefansson
Aug 26, 2014·International Journal of Pharmaceutics·Jawal SaidSimon Gaisford
Jan 1, 2013·Pathogens·David LebeauxChristophe Beloin
Sep 21, 2010·Expert Opinion on Pharmacotherapy·Philippe Paquet, Gérald E Piérard
Jul 17, 2015·Journal of Burn Care & Research : Official Publication of the American Burn Association·Linwood R HaithBruce H Ackerman
Jan 28, 2016·Diabetes/metabolism Research and Reviews·Edgar J Peters
Jul 28, 2012·The Journal of Surgical Research·Akhil K SethRobert D Galiano
Jul 24, 2012·Seminars in Vascular Surgery·Paul J Kim, John S Steinberg
Jun 19, 2012·Journal of the American College of Surgeons·Akhil K SethSeok J Hong
Mar 24, 2012·The Journal of Surgical Research·Aron G NusbaumStephen C Davis
Jul 5, 2011·Burns : Journal of the International Society for Burn Injuries·Merlin GuggenheimBernhard Guggenheim
Jan 29, 2011·Veterinary Microbiology·S J WestgateC A Cochrane
Sep 8, 2010·Journal of Microbiological Methods·Tom Coenye, Hans J Nelis
Nov 16, 2010·The Surgical Clinics of North America·Carrie E Black, J William Costerton
Apr 14, 2009·Veterinary Microbiology·E J WoodsS L Percival
Feb 24, 2009·Biomaterials·Evan M HetrickMark H Schoenfisch
Mar 17, 2015·Annals of Plastic Surgery·Krishna S Vyas, Lesley K Wong
Jul 25, 2009·The Journal of Investigative Dermatology·Jo M MartinGerald S Lazarus
May 20, 2014·Journal of Diabetes Research·Angela OatesAndrew J McBain
Jul 16, 2008·Current Opinion in Chemical Biology·Ian A Critchley, Urs A Ochsner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Related Papers

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Garth A JamesP S Stewart
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Thomas BjarnsholtMichael Givskov
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Steven L PercivalEmma Woods
Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Clark F SchierleRobert D Galiano
© 2021 Meta ULC. All rights reserved